J Physiol 210(2): 255-264
Glucose-induced electrical activity in pancreatic islet cells
Abstract
1. Intracellular recording of the transmembrane potential in mouse pancreatic cells revealed a membrane potential of -20·1 ± 0·8 mV for islet cells and -41·2 ± 1·4 mV for acinar cells.
2. The membrane potential of islet cells was glucose dependent and in the absence of glucose the cells hyperpolarized to -32·7 mV; with glucose 27·7 mM they depolarized to -16·1 mV.
3. Above a threshold concentration of glucose (4 mM) small action potentials of amplitude 1-4 mV were induced in islet cells. The percentage of cells impaled exhibiting action potentials reached a maximum of 80% at 27·7 mM glucose.
4. Mannose 16·6 mM was similar to glucose in its ability to induce action potential discharge in islet cells.
5. 2,4-Dinitrophenol (0·25 mM) hyperpolarized islet cells and blocked electrical activity induced by glucose 11·1 mM.
6. Adrenaline (1 μM) completely blocked glucose-induced electrical activity but without altering the membrane potential.
7. The origin and functional significance of glucose-induced electrical activity in islet cells is discussed in relation to insulin secretion.
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