Evidence for an increase in microviscosity of plasma membranes from soybean hypocotyls induced by the plant hormone, indole-3-acetic Acid.
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Journal: 2010/June - Plant Physiology
ISSN: 0032-0889
PUBMED: 16659715
Abstract:
The plant hormone indole-3-acetic acid (IAA or auxin) added at a concentration for half-maximal promotion of cell elongation (1 mum) caused an increase of 25% in the fluorescence polarization of the membrane-bound probe N-phenyl-1-naphthylamine, when added to fractions enriched in plasma membranes from soybean hypocotyls (Glycine max L. var. Wayne), with no measurable change in fluorescence lifetime. The amplitude of the polarization increase was maximal in the temperature range 12 to 22 C. The findings provide evidence for a cell-free response of isolated plasma membranes to the hormone and imply that the response involves an increase in the microviscosity of hydrocarbon regions of the membrane.
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Plant Physiol 58(4): 548-551
PMID: 16659715

Evidence for an Increase in Microviscosity of Plasma Membranes from Soybean Hypocotyls Induced by the Plant Hormone, Indole-3-Acetic Acid <sup><a href="#fn1" rid="fn1" class=" fn">1</a></sup>

Abstract

The plant hormone indole-3-acetic acid (IAA or auxin) added at a concentration for half-maximal promotion of cell elongation (1 μm) caused an increase of 25% in the fluorescence polarization of the membrane-bound probe N-phenyl-1-naphthylamine, when added to fractions enriched in plasma membranes from soybean hypocotyls (Glycine max L. var. Wayne), with no measurable change in fluorescence lifetime. The amplitude of the polarization increase was maximal in the temperature range 12 to 22 C. The findings provide evidence for a cell-free response of isolated plasma membranes to the hormone and imply that the response involves an increase in the microviscosity of hydrocarbon regions of the membrane.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
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Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907
This work was supported by National Institutes of Health General Medical Sciences Grant 18457, Research Career Development Award I KO4 29735 to W. A. C., National Institutes of Health Biophysics Traineeship to S. L. H., and National Institutes of Health Grant CA 13145 to D. J. M.
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Abstract
The plant hormone indole-3-acetic acid (IAA or auxin) added at a concentration for half-maximal promotion of cell elongation (1 μm) caused an increase of 25% in the fluorescence polarization of the membrane-bound probe N-phenyl-1-naphthylamine, when added to fractions enriched in plasma membranes from soybean hypocotyls (Glycine max L. var. Wayne), with no measurable change in fluorescence lifetime. The amplitude of the polarization increase was maximal in the temperature range 12 to 22 C. The findings provide evidence for a cell-free response of isolated plasma membranes to the hormone and imply that the response involves an increase in the microviscosity of hydrocarbon regions of the membrane.
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