The present study was carried out to compare the subgingival plaque microflora in mango leaf users. Fifty subjects of both sexes, 25 of them used tooth brush and 25 used mango leaf as their home care hygiene device were included in the study. The microbiological evaluation for specific bacterial counts of Actinobacillus actinomycetemcomitans, Prevotella intermedia, Porphyromonas gingivalis, Fusobacterium nucleatum and Peptostreptococcus micros were carried out for all subject. Specific microbial evaluation revealed significant decrease in the proportion of P. intermedia and P. gingivalis in mango leaf users compared to tooth brush users. It shows that mangiferin possesses antibacterial activity in vivo against specific periodontal pathogens such as P. intermedia and P. gingivalis. Use of mango leaf in conjunction with a tooth brush will be a good home care device for maintenance of oral hygiene.