Abstract:

We have developed a simple and efficient transformation system for the agaric fungus, Coprinus cinereus. Protoplasts were prepared from asexual spores that harbor one or two mutations in the structural gene for tryptophan synthetase. The protoplasts can be stably transformed using the cloned Coprinus gene at a frequency of 1 in 10(4) viable protoplasts. A variety of molecular events accompanies the formation of stable transformants, including insertion of the transforming DNA at the homologous locus. The transforming DNA is stable through cell division, mating, fruiting body formation, and meiosis.

Open in

PUBMED | PMC | Google Scholar | Wikipedia

Relations:

Content

Citations

(77)

References

(28)

Drugs

(1)

Chemicals

(1)

Organisms

(2)

Processes

(3)

DNA-mediated transformation of the basidiomycete Coprinus cinereus.

Abstract

We have developed a simple and efficient transformation system for the agaric fungus, Coprinus cinereus. Protoplasts were prepared from asexual spores that harbor one or two mutations in the structural gene for tryptophan synthetase. The protoplasts can be stably transformed using the cloned Coprinus gene at a frequency of 1 in 10(4) viable protoplasts. A variety of molecular events accompanies the formation of stable transformants, including insertion of the transforming DNA at the homologous locus. The transforming DNA is stable through cell division, mating, fruiting body formation, and meiosis.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.2M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Fig. 2.
on p.837

Fig. 3.
on p.837

Fig. 4.
on p.838

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

Ballance DJ, Turner G. Development of a high-frequency transforming vector for Aspergillus nidulans. Gene. 1985;36(3):321–331. [PubMed] [Google Scholar]
Ballance DJ, Buxton FP, Turner G. Transformation of Aspergillus nidulans by the orotidine-5'-phosphate decarboxylase gene of Neurospora crassa. Biochem Biophys Res Commun. 1983 Apr 15;112(1):284–289. [PubMed] [Google Scholar]
Case ME. Genetical and molecular analyses of qa-2 transformants in Neurospora crassa. Genetics. 1986 Jul;113(3):569–587.[PMC free article] [PubMed] [Google Scholar]
Case ME, Schweizer M, Kushner SR, Giles NH. Efficient transformation of Neurospora crassa by utilizing hybrid plasmid DNA. Proc Natl Acad Sci U S A. 1979 Oct;76(10):5259–5263.[PMC free article] [PubMed] [Google Scholar]
Dagert M, Ehrlich SD. Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells. Gene. 1979 May;6(1):23–28. [PubMed] [Google Scholar]
Ish-Horowicz D, Burke JF. Rapid and efficient cosmid cloning. Nucleic Acids Res. 1981 Jul 10;9(13):2989–2998.[PMC free article] [PubMed] [Google Scholar]
Kelly JM, Hynes MJ. Transformation of Aspergillus niger by the amdS gene of Aspergillus nidulans. EMBO J. 1985 Feb;4(2):475–479.[PMC free article] [PubMed] [Google Scholar]
Kingsman AJ, Gimlich RL, Clarke L, Chinault AC, Carbon J. Sequence variation in dispersed repetitive sequences in Saccharomyces cerevisiae. J Mol Biol. 1981 Feb 5;145(4):619–632. [PubMed] [Google Scholar]
Lin FL, Sperle K, Sternberg N. Recombination in mouse L cells between DNA introduced into cells and homologous chromosomal sequences. Proc Natl Acad Sci U S A. 1985 Mar;82(5):1391–1395.[PMC free article] [PubMed] [Google Scholar]
Lu BC, Raju NB. Meiosis in Coprinus. II. Chromosome pairing and the lampbrush diplotene stage of meiotic prophase. Chromosoma. 1970;29(3):305–316. [PubMed] [Google Scholar]
Munoz-Rivas A, Specht CA, Drummond BJ, Froeliger E, Novotny CP, Ullrich RC. Transformation of the basidiomycete, Schizophyllum commune. Mol Gen Genet. 1986 Oct;205(1):103–106. [PubMed] [Google Scholar]
Orr-Weaver TL, Szostak JW, Rothstein RJ. Yeast transformation: a model system for the study of recombination. Proc Natl Acad Sci U S A. 1981 Oct;78(10):6354–6358.[PMC free article] [PubMed] [Google Scholar]
Pukkila PJ, Lu BC. Silver staining of meiotic chromosomes in the fungus, Coprinus cinereus. Chromosoma. 1985;91(2):108–112. [PubMed] [Google Scholar]
Rao PS, Niederpruem DJ. Carbohydrate metabolism during morphogenesis of Coprinus lagopus (sensu Buller). J Bacteriol. 1969 Dec;100(3):1222–1228.[PMC free article] [PubMed] [Google Scholar]
Rigby PW, Dieckmann M, Rhodes C, Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. [PubMed] [Google Scholar]
Shortle D, Haber JE, Botstein D. Lethal disruption of the yeast actin gene by integrative DNA transformation. Science. 1982 Jul 23;217(4557):371–373. [PubMed] [Google Scholar]
Smith AJ, Berg P. Homologous recombination between defective neo genes in mouse 3T6 cells. Cold Spring Harb Symp Quant Biol. 1984;49:171–181. [PubMed] [Google Scholar]
Thomas KR, Folger KR, Capecchi MR. High frequency targeting of genes to specific sites in the mammalian genome. Cell. 1986 Feb 14;44(3):419–428. [PubMed] [Google Scholar]
Tilburn J, Scazzocchio C, Taylor GG, Zabicky-Zissman JH, Lockington RA, Davies RW. Transformation by integration in Aspergillus nidulans. Gene. 1983 Dec;26(2-3):205–221. [PubMed] [Google Scholar]
Tilby MJ. Tryptophan biosynthesis in Coprinus lagopus: a genetic analysis of mutants. J Gen Microbiol. 1976 Mar;93(1):126–132. [PubMed] [Google Scholar]
Umbarger HE. Amino acid biosynthesis and its regulation. Annu Rev Biochem. 1978;47:532–606. [PubMed] [Google Scholar]
Messing J, Vieira J. A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments. Gene. 1982 Oct;19(3):269–276. [PubMed] [Google Scholar]
Walz A, Ratzkin B, Carbon J. Control of expression of a cloned yeast (Saccharomyces cerevisiae) gene (trp5) by a bacterial insertion element (IS2). Proc Natl Acad Sci U S A. 1978 Dec;75(12):6172–6176.[PMC free article] [PubMed] [Google Scholar]
Woodward VW, De Zeeuw JR, Srb AM. THE SEPARATION AND ISOLATION OF PARTICULAR BIOCHEMICAL MUTANTS OF NEUROSPORA BY DIFFERENTIAL GERMINATION OF CONIDIA, FOLLOWED BY FILTRATION AND SELECTIVE PLATING. Proc Natl Acad Sci U S A. 1954 Mar;40(3):192–200.[PMC free article] [PubMed] [Google Scholar]
Yelton MM, Hamer JE, Timberlake WE. Transformation of Aspergillus nidulans by using a trpC plasmid. Proc Natl Acad Sci U S A. 1984 Mar;81(5):1470–1474.[PMC free article] [PubMed] [Google Scholar]
Yelton MM, Timberlake WE, Hondel CA. A cosmid for selecting genes by complementation in Aspergillus nidulans: Selection of the developmentally regulated yA locus. Proc Natl Acad Sci U S A. 1985 Feb;82(3):834–838.[PMC free article] [PubMed] [Google Scholar]
Zalkin H, Yanofsky C. Yeast gene TRP5: structure, function, regulation. J Biol Chem. 1982 Feb 10;257(3):1491–1500. [PubMed] [Google Scholar]
Zolan ME, Pukkila PJ. Inheritance of DNA methylation in Coprinus cinereus. Mol Cell Biol. 1986 Jan;6(1):195–200.[PMC free article] [PubMed] [Google Scholar]

Copyright notice

Abstract

We have developed a simple and efficient transformation system for the agaric fungus, Coprinus cinereus. Protoplasts were prepared from asexual spores that harbor one or two mutations in the structural gene for tryptophan synthetase. The protoplasts can be stably transformed using the cloned Coprinus gene at a frequency of 1 in 10(4) viable protoplasts. A variety of molecular events accompanies the formation of stable transformants, including insertion of the transforming DNA at the homologous locus. The transforming DNA is stable through cell division, mating, fruiting body formation, and meiosis.

Collaboration tool especially designed for Life Science professionals.Drag-and-drop any entity to your messages.

Learn More