Scrophularia mottle virus (ScMV) was newly found in the Czech Republic in Scrophularia nodosa L. plants. The Czech isolate (ScMV-C) was serologically identical and similar in symptoms and host range to the Italian Anagyris strain (ScMV-I). Nicotiana tabacum L. cv. Samsun, Nicotiana glutinosa L., Nicotiana tabacum L. cv. White Burley, Physalis floridana Rybd. and Cucumis sativus L. are described as new host plants of ScMV. Double-stranded RNA patterns and the isoelectric point of this virus are characterized.