OBJECTIVE

To study the chemical constituents of Pleione bulbocodioides.

METHODS

The compounds were isolated by repeated column chromatography with silica gel, Sephadex LH-20 and ODS-HPLC. The structures were elucidated by analysis of spectroscopic data (1H-NMR, 13C-NMR).

RESULTS

Nine compounds were isolated from the EtOAc extract in the rhizoma of Pleione bulbocodioides. Their structures were identified as amentoflavone(I), kayaflavone(II), gymconopin D(III), methyl(4-OH) phenylacetate (IV), p-hydroxy benzaldehyde(V), p-hydroxybenzcic acid (VI), 4-oxopentanoic acid (VII), p-dihydroxy benzene (VIII), gastrodine (IX).

CONCLUSIONS

Compounds I - IX are isolated from this plant for the first time.