Biochem J 170(3): 615-625
Calcium metabolism in rat hepatocytes.
Abstract
1. The total calcium concentration in rat hepatocytes was 7.9 microgram-atoms/g dry wt.; 77% of this was mitochondrial. Approx. 20% of cell calcium exchanged with 45Ca within 2 min. Thereafter incorporation proceeded at a low rate to reach 28% of total calcium after 60 min. Incorporation into mitochondria showed a similar time course and accounted for 20% of mitochondrial total calcium after 60 min. 2. The alpha-adrenergic agonists phenylephrine and adrenaline + propranolol stimulated incorporation of 45Ca into hepatocytes. Phenylephrine was shown to increase total calcium in hepatocytes. Phenylephrine inhibited efflux fo 45Ca from hepatocytes perifused with calcium-free medium. 3. Glucagon, dibutryl cyclic AMP and beta-adrenergic agonists adrenaline and 3-isobutyl-1-methyl-xanthine stimulated calcium efflux from hepatocytes perifused with calcium-free medium. The effect of glucagon was blocked by insulin. Insulin itself had no effect on calcium efflux and it did not affect the response to dibutyryl cyclic AMP. 4. Incorporation of 45Ca into mitochondria in hepatocytes was stimulated by phenylephrine and inhibited by glucagon and by carbonyl cyanide p-trifluoromethoxyphenylhydrazone. The effect of glucagon was blocked by insulin. 5. Ionophore A23187 stimulated hepatocyte uptake of 45Ca, uptake of 45Ca into mitochondria in hepatocytes and efflux of 45Ca into a calcium-free medium.
Full text
Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.6M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Assimacopoulos-Jeannet FD, Blackmore PF, Exton JH. Studies on alpha-adrenergic activation of hepatic glucose output. Studies on role of calcium in alpha-adrenergic activation of phosphorylase. J Biol Chem. 1977 Apr 25;252(8):2662–2669. [PubMed] [Google Scholar]
- Berry MN, Friend DS. High-yield preparation of isolated rat liver parenchymal cells: a biochemical and fine structural study. J Cell Biol. 1969 Dec;43(3):506–520.[PMC free article] [PubMed] [Google Scholar]
- Birnbaum MJ, Fain JN. Activation of protein kinase and glycogen phosphorylase in isolated rat liver cells by glucagon and catecholamines. J Biol Chem. 1977 Jan 25;252(2):528–535. [PubMed] [Google Scholar]
- Chen RF. Removal of fatty acids from serum albumin by charcoal treatment. J Biol Chem. 1967 Jan 25;242(2):173–181. [PubMed] [Google Scholar]
- Cherrington AD, Assimacopoulos FD, Harper SC, Corbin JD, Park CR, Exton JH. Studies on the alpha-andrenergic activation of hepatic glucose output. II. Investigation of the roles of adenosine 3':5'-monophosphate and adenosine 3':5'-monophosphate-dependent protein kinase in the actions of phenylephrine in isolated hepatocytes. J Biol Chem. 1976 Sep 10;251(17):5209–5218. [PubMed] [Google Scholar]
- Coore HG, Denton RM, Martin BR, Randle PJ. Regulation of adipose tissue pyruvate dehydrogenase by insulin and other hormones. Biochem J. 1971 Nov;125(1):115–127.[PMC free article] [PubMed] [Google Scholar]
- Cornell NW, Lund P, Hems R, Krebs HA. Acceleration of gluconeogenesis from lactate by lysine (Short Communication). Biochem J. 1973 Jun;134(2):671–672.[PMC free article] [PubMed] [Google Scholar]
- Exton JH, Harper SC. Role of cyclic AMP in the actions of catecholamines on hepatic carbohydrate metabolism. Adv Cyclic Nucleotide Res. 1975;5:519–532. [PubMed] [Google Scholar]
- Exton JH, Robison GA, Sutherland EW, Park CR. Studies on the role of adenosine 3',5'-monophosphate in the hepatic actions of glucagon and catecholamines. J Biol Chem. 1971 Oct 25;246(20):6166–6177. [PubMed] [Google Scholar]
- Friedmann N, Park CR. Early effects of 3',5'-adenosine monophosphate on the fluxes of calcium end potassium in the perfused liver of normal and adrenalectomized rats. Proc Natl Acad Sci U S A. 1968 Oct;61(2):504–508.[PMC free article] [PubMed] [Google Scholar]
- Hodârnău A, Dancea S, Bârzu O. Isolation of highly purified mitochondria from rat pancreas. J Cell Biol. 1973 Oct;59(1):222–227.[PMC free article] [PubMed] [Google Scholar]
- Hutson NJ, Brumley FT, Assimacopoulos FD, Harper SC, Exton JH. Studies on the alpha-adrenergic activation of hepatic glucose output. I. Studies on the alpha-adrenergic activation of phosphorylase and gluconeogenesis and inactivation of glycogen synthase in isolated rat liver parenchymal cells. J Biol Chem. 1976 Sep 10;251(17):5200–5208. [PubMed] [Google Scholar]
- Kanagasuntheram P, Randle PJ. Calcium metabolism and amylase release in rat parotid acinar cells. Biochem J. 1976 Dec 15;160(3):547–564.[PMC free article] [PubMed] [Google Scholar]
- Keppens S, Vandenheede JR, De Wulf H. On the role of calcium as second messenger in liver for the hormonally induced activation of glycogen phosphorylase. Biochim Biophys Acta. 1977 Feb 28;496(2):448–457. [PubMed] [Google Scholar]
- Pilkis SJ, Claus TH, Johnson RA, Park CR. Hormonal control of cyclic 3':5'-AMP levels and gluconeogenesis in isolated hepatocytes from fed rats. J Biol Chem. 1975 Aug 25;250(16):6328–6336. [PubMed] [Google Scholar]
- Randle PJ, Denton RM, Pask HT, Severson DL. Calcium ions and the regulation of pyruvate dehydrogenase. Biochem Soc Symp. 1974;(39):75–88. [PubMed] [Google Scholar]
- Reed KC. Binding of calcium by cellulose membranes and sephadex. Biochem Biophys Res Commun. 1973 Feb 20;50(4):1136–1142. [PubMed] [Google Scholar]
- Reed PW, Lardy HA. A23187: a divalent cation ionophore. J Biol Chem. 1972 Nov 10;247(21):6970–6977. [PubMed] [Google Scholar]
- Schotland J, Mela L. Role of cyclic nucleotides in the regulation of mitochondrial calcium uptake and efflux kinetics. Biochem Biophys Res Commun. 1977 Apr 25;75(4):920–924. [PubMed] [Google Scholar]
- Severson DL, Denton RM, Pask HT, Randle PJ. Calcium and magnesium ions as effectors of adipose-tissue pyruvate dehydrogenase phosphate phosphatase. Biochem J. 1974 May;140(2):225–237.[PMC free article] [PubMed] [Google Scholar]
- Severson DL, Denton RM, Bridges BJ, Randle PJ. Exchangeable and total calcium pools in mitochondria of rat epididymal fat-pads and isolated fat-cells. Role in the regulation of pyruvate dehydrogenase activity. Biochem J. 1976 Jan 15;154(1):209–223.[PMC free article] [PubMed] [Google Scholar]
- Sherline P, Lynch A, Glinsmann WH. Cyclic AMP and adrenergic receptor control of rat liver glycogen metabolism. Endocrinology. 1972 Sep;91(3):680–690. [PubMed] [Google Scholar]
- Stanley PE, Williams SG. Use of the liquid scintillation spectrometer for determining adenosine triphosphate by the luciferase enzyme. Anal Biochem. 1969 Jun;29(3):381–392. [PubMed] [Google Scholar]
- van de Werve G, Hue L, Hers HG. Hormonal and ionic control of the glycogenolytic cascade in rat liver. Biochem J. 1977 Jan 15;162(1):135–142.[PMC free article] [PubMed] [Google Scholar]
- Wilson DF, Stubbs M, Veech RL, Erecińska M, Krebs HA. Equilibrium relations between the oxidation-reduction reactions and the adenosine triphosphate synthesis in suspensions of isolated liver cells. Biochem J. 1974 Apr;140(1):57–64.[PMC free article] [PubMed] [Google Scholar]