Bioassay-guided isolation of anti-seizure principles from Semen Pharbitidis using a zebrafish pentylenetetrazol seizure model.
Journal: 2018/December - Journal of Ethnopharmacology
ISSN: 1872-7573
PUBMED: 30572093
Abstract:Semen Pharbitidis, the seeds of Pharbitis nil (Linn.) Choisy (Convolvulaceae) is a well-known traditional Chinese medicinal plant used for treating helminthiasis and epilepsy in China.This study aims to identify the anti-seizure components from Semen Pharbitidis.A bioassay-guided isolation of anti-seizure compounds from Semen Pharbitidis was performed using a zebrafish pentylenetetrazol seizure model. The structures of active compounds were elucidated by high resolution mass spectrometry. The fragments of active compounds were tested for anti-seizure activity as well.The bioassay-guided isolation of ethanol extract of Semen Pharbitidis led to a group of resin glucosides, namely pharbitin. One of the fragments of pharbitin, 2-methylbutyric acid, also showed anti-seizure activity.We provided further experimental scientific evidence to support the traditional use of Semen Pharbitidis for the treatment of epilepsy. Pharbitin was identified to be the main anti-seizure component in Semen Pharbitidis.
Open in
Relations:
Citations
(1)
Diseases
(2)
Drugs
(1)
Chemicals
(1)
Organisms
(2)
Anatomy
(2)
Affiliates
(1)
Similar articles
Discussion board
Collaboration tool especially designed for Life Science professionals.Drag-and-drop any entity to your messages.
Search and select entities to see details here. Details will include lists of related entities (via publication). Explore related entities further.