Antimicrobial substance from a human Lactobacillus strain.
Abstract
Lactobacillus sp. strain GG, which was isolated from the feces of a normal person, produced a substance with potent inhibitory activity against a wide range of bacterial species. It inhibited anaerobic bacteria (Clostridium spp., Bacteroides spp., Bifidobacterium spp.), members of the family Enterobacteriaceae, Pseudomonas spp. Staphylococcus spp., and Streptococcus spp., as demonstrated by a microbiological assay; however, it did not inhibit other lactobacilli. The inhibitory activity occurred between pH 3 and 5 and was heat stable. Bactericidal activity against Escherichia coli was demonstrated at a dilution of 1:128. The inhibitory substance was distinct from lactic and acetic acids. It had a low molecular weight (less than 1,000) and was soluble in acetone-water (10:1). Because of these characteristics, the inhibitory material could not be considered a bacteriocin; it most closely resembled a microcin, which has been associated previously with members of the family Enterobacteriaceae.
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- Asensio C, Pérez-Díaz JC. A new family of low molecular weight antibiotics from enterobacteria. Biochem Biophys Res Commun. 1976 Mar 8;69(1):7–14. [PubMed] [Google Scholar]
- Barefoot SF, Klaenhammer TR. Purification and characterization of the Lactobacillus acidophilus bacteriocin lactacin B. Antimicrob Agents Chemother. 1984 Sep;26(3):328–334.[PMC free article] [PubMed] [Google Scholar]
- BECK C, NECHELES H. Beneficial effects of administration of Lactobacillus acidophilus in diarrheal and other intestinal disorders. Am J Gastroenterol. 1961 May;35:522–530. [PubMed] [Google Scholar]
- DE KLERK HC, COETZEE JN. Antibiosis among lactobacilli. Nature. 1961 Oct 28;192:340–341. [PubMed] [Google Scholar]
- Deneke CF, McGowan K, Thorne GM, Gorbach SL. Attachment of enterotoxigenic Escherichia coli to human intestinal cells. Infect Immun. 1983 Mar;39(3):1102–1106.[PMC free article] [PubMed] [Google Scholar]
- Goldin B, Gorbach SL. Alterations in fecal microflora enzymes related to diet, age, lactobacillus supplements, and dimethylhydrazine. Cancer. 1977 Nov;40(5 Suppl):2421–2426. [PubMed] [Google Scholar]
- Goldin BR, Gorbach SL. Effect of Lactobacillus acidophilus dietary supplements on 1,2-dimethylhydrazine dihydrochloride-induced intestinal cancer in rats. J Natl Cancer Inst. 1980 Feb;64(2):263–265. [PubMed] [Google Scholar]
- Hamdan IY, Mikolajcik EM. Acidolin: an antibiotic produced by Lactobacillus acidophilus. J Antibiot (Tokyo) 1974 Aug;27(8):631–636. [PubMed] [Google Scholar]
- Schumacher GF, Schill WB. Radial diffusion in gel for micro determination of enzymes. II. Plasminogen activator, elastase, and nonspecific proteases. Anal Biochem. 1972 Jul;48(1):9–26. [PubMed] [Google Scholar]
- Speck ML. Interactions among lactobacilli and man. J Dairy Sci. 1976 Feb;59(2):338–343. [PubMed] [Google Scholar]
- Tagg JR, Dajani AS, Wannamaker LW. Bacteriocins of gram-positive bacteria. Bacteriol Rev. 1976 Sep;40(3):722–756.[PMC free article] [PubMed] [Google Scholar]
- VINCENT JG, VEOMETT RC, RILEY RF. Antibacterial activity associated with Lactobacillus acidophilus. J Bacteriol. 1959 Oct;78:477–484.[PMC free article] [PubMed] [Google Scholar]