Antimicrob Agents Chemother 37(12): 2534-2539
Antimicrobial activity of rabbit CAP18-derived peptides.
Abstract
A cationic antimicrobial protein of 18 kDa (CAP18) was originally isolated from rabbit granulocytes by using as an assay the agglutination of Re-lipopolysaccharide-coated erythrocytes. The C-terminal 37 amino acids of CAP18 (CAP18(106-142)) make up the lipopolysaccharide-binding domain. Synthetic CAP18(106-142) has broad antimicrobial activity against both gram-positive (50% inhibitory concentration, 130 to 200 nM) and gram-negative (50% inhibitory concentration, 20 to 100 nM) bacteria. Susceptible strains include Staphylococcus aureus, Streptococcus pneumoniae, Escherichia coli, Pseudomonas aeruginosa, and Salmonella typhimurium. Antimicrobial activity is highly dependent on peptide structure. Although a 32-amino-acid peptide resulting from the truncation of 5 amino acids from the C terminus of CAP18(106-142) is highly active, other fragments of CAP18(106-142), including CAP18(106-142) with a truncated N terminus, do not exhibit antimicrobial activity. Unlike previously characterized antimicrobial peptides derived from granulocyte proteins, CAP18(106-142) is active in serum. CAP18(106-142) or a derivative peptide may have therapeutic potential for bacterial sepsis.
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- Brade L, Brade H, Fischer W. A 28 kDa protein of normal mouse serum binds lipopolysaccharides of gram-negative and lipoteichoic acids of gram-positive bacteria. Microb Pathog. 1990 Nov;9(5):355–362. [PubMed] [Google Scholar]
- Del Sal G, Storici P, Schneider C, Romeo D, Zanetti M. cDNA cloning of the neutrophil bactericidal peptide indolicidin. Biochem Biophys Res Commun. 1992 Aug 31;187(1):467–472. [PubMed] [Google Scholar]
- Gazzano-Santoro H, Parent JB, Grinna L, Horwitz A, Parsons T, Theofan G, Elsbach P, Weiss J, Conlon PJ. High-affinity binding of the bactericidal/permeability-increasing protein and a recombinant amino-terminal fragment to the lipid A region of lipopolysaccharide. Infect Immun. 1992 Nov;60(11):4754–4761.[PMC free article] [PubMed] [Google Scholar]
- Hirata M, Yoshida M, Inada K, Kirikae T. Investigation of endotoxin binding cationic proteins from granulocytes; agglutination of erythrocytes sensitized with Re-LPS. Adv Exp Med Biol. 1990;256:287–299. [PubMed] [Google Scholar]
- Larrick JW, Morgan JG, Palings I, Hirata M, Yen MH. Complementary DNA sequence of rabbit CAP18--a unique lipopolysaccharide binding protein. Biochem Biophys Res Commun. 1991 Aug 30;179(1):170–175. [PubMed] [Google Scholar]
- Lehrer RI, Lichtenstein AK, Ganz T. Defensins: antimicrobial and cytotoxic peptides of mammalian cells. Annu Rev Immunol. 1993;11:105–128. [PubMed] [Google Scholar]
- Marra MN, Wilde CG, Collins MS, Snable JL, Thornton MB, Scott RW. The role of bactericidal/permeability-increasing protein as a natural inhibitor of bacterial endotoxin. J Immunol. 1992 Jan 15;148(2):532–537. [PubMed] [Google Scholar]
- Pereira HA, Erdem I, Pohl J, Spitznagel JK. Synthetic bactericidal peptide based on CAP37: a 37-kDa human neutrophil granule-associated cationic antimicrobial protein chemotactic for monocytes. Proc Natl Acad Sci U S A. 1993 May 15;90(10):4733–4737.[PMC free article] [PubMed] [Google Scholar]
- Ritonja A, Kopitar M, Jerala R, Turk V. Primary structure of a new cysteine proteinase inhibitor from pig leucocytes. FEBS Lett. 1989 Sep 25;255(2):211–214. [PubMed] [Google Scholar]
- Shafer WM, Martin LE, Spitznagel JK. Cationic antimicrobial proteins isolated from human neutrophil granulocytes in the presence of diisopropyl fluorophosphate. Infect Immun. 1984 Jul;45(1):29–35.[PMC free article] [PubMed] [Google Scholar]
- Vaara M. Agents that increase the permeability of the outer membrane. Microbiol Rev. 1992 Sep;56(3):395–411.[PMC free article] [PubMed] [Google Scholar]
- Weiss J, Elsbach P, Shu C, Castillo J, Grinna L, Horwitz A, Theofan G. Human bactericidal/permeability-increasing protein and a recombinant NH2-terminal fragment cause killing of serum-resistant gram-negative bacteria in whole blood and inhibit tumor necrosis factor release induced by the bacteria. J Clin Invest. 1992 Sep;90(3):1122–1130.[PMC free article] [PubMed] [Google Scholar]