Biochem J 178(1): 249-251
Adenosine, thyroid status and regulation of lipolysis.
Abstract
Adipocytes from hypothyroid rats do not respond to adrenaline with increased glycerol release. Adenosine deaminase largely restores lipolytic sensitivity. This effect is reversed by 2-deoxycoformycin, an inhibitor of the enzyme, and by N6-(phenylisopropyl)adenosine, which is not deaminated. Lipolytic response of normal cells to adrenaline is only 50% inhibited by phenylisopropyladenosine, whereas in cells from hypothyroid rats blockage is total. Inhibition of 50% was seen at 100 and 1 nM concentrations respectively. Insensitivity to adrenaline of hypothyroid-rat adipocytes can, at least partly, be explained by increased sensitivity to adenosine.
Full text
Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (440K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Armstrong KJ, Stouffer JE, Van Inwegen RG, Thompson WJ, Robison GA. Effects of thyroid hormone deficiency on cyclic adenosine 3':5'-monophosphate and control of lipolysis in fat cells. J Biol Chem. 1974 Jul 10;249(13):4226–4231. [PubMed] [Google Scholar]
- Ciaraldi T, Marinetti GV. Thyroxine and propylthiouracil effects of vivo on alpha and beta adrenergic receptors in rat heart. Biochem Biophys Res Commun. 1977 Feb 7;74(3):984–991. [PubMed] [Google Scholar]
- Correze C, Laudat MH, Laudat P, Nunez J. Hormone-dependent lipolysis in fat-cells from thyroidectomized rats. Mol Cell Endocrinol. 1974 Oct;1(5):309–327. [PubMed] [Google Scholar]
- DOLE VP. Effect of nucleic acid metabolites on lipolysis in adipose tissue. J Biol Chem. 1961 Dec;236:3125–3130. [PubMed] [Google Scholar]
- Ebert R, Schwabe U. Studies on the antilipolytic effect of adenosine and related compounds in isolated fat cells. Naunyn Schmiedebergs Arch Pharmacol. 1973;278(3):247–259. [PubMed] [Google Scholar]
- Fain JN. Inhibition of adenosine cyclic 3', 5'-monophosphate accumulation in fat cells by adenosine, N6-(phenylisopropyl) adenosine, and related compounds. Mol Pharmacol. 1973 Sep;9(5):595–604. [PubMed] [Google Scholar]
- Fain JN, Wieser PB. Effects of adenosine deaminase on cyclic adenosine monophosphate accumulation, lipolysis, and glucose metabolism of fat cells. J Biol Chem. 1975 Feb 10;250(3):1027–1034. [PubMed] [Google Scholar]
- Fain JN, Pointer RH, Ward WF. Effects of adenosine nucleosides on adenylate cyclase, phosphodiesterase, cyclic adenosine monophosphate accumulation, and lipolysis in fat cells. J Biol Chem. 1972 Nov 10;247(21):6866–6872. [PubMed] [Google Scholar]
- Fernandez BM, Saggerson ED. Alterations in response of rat white adipocytes to insulin, noradrenaline, corticotropin and glucagon after adrenalectomy. Correction of these changes by adenosine deaminase. Biochem J. 1978 Jul 15;174(1):111–118.[PMC free article] [PubMed] [Google Scholar]
- Harwood JP, Löw H, Rodbell M. Stimulatory and inhibitory effects of guanyl nucleotides on fat cell adenylate cyclase. J Biol Chem. 1973 Sep 10;248(17):6239–6245. [PubMed] [Google Scholar]
- Ichikawa A, Matsumoto H, Sakato N, Tomita K. Effect of thyroid hormones on epinephrine-induced lipolysis in adipose tissue of rats. J Biochem. 1971 Jun;69(6):1055–1064. [PubMed] [Google Scholar]
- Malbon CC, Moreno FJ, Cabelli RJ, Fain JN. Fat cell adenylate cyclase and beta-adrenergic receptors in altered thyroid states. J Biol Chem. 1978 Feb 10;253(3):671–678. [PubMed] [Google Scholar]
- Raben MS, Matsuzaki F. Effect of purines on epinephrine-induced lipolysis in adipose tissue. J Biol Chem. 1966 Oct 25;241(20):4781–4786. [PubMed] [Google Scholar]
- Sawa T, Fukagawa Y, Homma I, Takeuchi T, Umezawa H. Mode of inhibition of coformycin on adenosine deaminase. J Antibiot (Tokyo) 1967 Jul;20(4):227–231. [PubMed] [Google Scholar]
- Schwabe U, Ebert R, Erbler HC. Adenosine release from isolated fat cells and its significance for the effects of hormones on cyclic 3',5'-AMP levels and lipolysis. Naunyn Schmiedebergs Arch Pharmacol. 1973;276(2):133–148. [PubMed] [Google Scholar]
- Schwabe U, Schönhöfer PS, Ebert R. Facilitation by adenosine of the action of insulin on the accumulation of adenosine 3':5'-monophosphate, lipolysis, and glucose oxidation in isolated fat cells. Eur J Biochem. 1974 Aug 1;46(3):537–545. [PubMed] [Google Scholar]
- Vaughan M. An in vitro effect of triiodothyronine on rat adipose tissue. J Clin Invest. 1967 Sep;46(9):1482–1491.[PMC free article] [PubMed] [Google Scholar]
- Williams LT, Lefkowitz RJ, Watanabe AM, Hathaway DR, Besch HR., Jr Thyroid hormone regulation of beta-adrenergic receptor number. J Biol Chem. 1977 Apr 25;252(8):2787–2789. [PubMed] [Google Scholar]