Desiccation and Cold Hardening of Date Palm Somatic Embryos Improve Germination.
Journal: 2017/July - Methods in Molecular Biology
ISSN: 1940-6029
Abstract:
Embryogenic suspension cultures of date palm are ideal for mass propagation of somatic embryos; however, the low percentage of germination of somatic embryos (SE) remains an impediment. This chapter focuses on two important physical factors to improve germination of date palm somatic embryos: the use of partial desiccation (3 h) of somatic embryos and the exposure to low temperature (4 °C for 24 h). High germination percentage (41%) is achieved by desiccation for 3 h. Moreover, adding 0.3 g/L activated charcoal (AC) to the liquid medium further improves somatic embryo number and weight as well as the percentage of germination. Moreover, partial desiccation and low temperature exposure tend to increase proline content. This improved protocol for somatic embryo germination is potentially applicable for commercial micropropagation of date palm.
Relations:
Drugs
(1)
Affiliates
(1)
Similar articles
Articles by the same authors
Discussion board
Collaboration tool especially designed for Life Science professionals.Drag-and-drop any entity to your messages.