Plant Physiol 52(6): 549-553
The Effects of Octanoate and Oleate on Isocitrate Lyase Activity during the Germination of <em>Pinus pinea</em> Seeds <sup><a href="#fn1" rid="fn1" class=" fn">1</a></sup>
Abstract
The changes of isocitrate lyase levels with respect to the catabolism of triglycerides have been studied during the germination of Pinus pinea seeds. We studied the effects of octanoate, oleate, and inhibitors of protein synthesis on isocitrate lyase during germination. Pyruvate kinase, glucose-6-P-dehydrogenase, malate dehydrogenase, and isocitrate dehydrogenase were also assayed. Octanoate and oleate inhibited the isocitrate lyase activity, similarly to cycloheximide, chloramphenicol, and actinomycin, inhibitors of protein biosynthesis. This inhibitory effect is not specific but is strikingly evident with isocitrate lyase. This inhibition was not proportional to the concentration but was proportional to the chain length of oleate and octanoate.
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- BEEVERS H. Metabolic production of sucrose from fat. Nature. 1961 Jul 29;191:433–436. [PubMed] [Google Scholar]
- BRADBEER C, STUMPF PK. Fat metabolism in higher plants. XI. The conversion of fat into carbohydrate in peanut and sunflower seedlings. J Biol Chem. 1959 Mar;234(3):498–501. [PubMed] [Google Scholar]
- Carpenter WD, Beevers H. Distribution and Properties of Isocitritase in Plants. Plant Physiol. 1959 Jul;34(4):403–409.[PMC free article] [PubMed] [Google Scholar]
- Ching TM. Glyoxysomes in megagamethophyte of germinating ponderosa pine seeds. Plant Physiol. 1970 Sep;46(3):475–482.[PMC free article] [PubMed] [Google Scholar]
- Daron HH, Rutter WJ, Cunsalus IC. Isocitrate lyase. Kinetics and substrate-tritium exchange reactions. Biochemistry. 1966 Mar;5(3):895–903. [PubMed] [Google Scholar]
- Filner P, Varner JE, Wray JL. Environmental or developmental changes cause many enzyme activities of higher plants to rise or fall. Science. 1969 Jul 25;165(3891):358–367. [PubMed] [Google Scholar]
- Firenzuoli AM, Vanni P, Mastronuzzi E, Zanobini A, Baccari V. Participation of the glyoxylate cycle in the metabolism of germinating seed of Pinus pinca. Life Sci. 1968 Dec 15;7(24):1251–1258. [PubMed] [Google Scholar]
- Firenzuoli AM, Vanni P, Mastronuzzi E, Zanobini A, Baccari V. Enzymes of glyoxylate in conifers. Plant Physiol. 1968 Jul;43(7):1125–1128.[PMC free article] [PubMed] [Google Scholar]
- Firenzuoli AM, Vanni P, Ramponi G, Baccari V. Changes in Enzyme Levels During Germination of Seeds of Triticum durum. Plant Physiol. 1968 Feb;43(2):260–264.[PMC free article] [PubMed] [Google Scholar]
- Gibbs M, Beevers H. Glucose Dissimilation in the Higher Plant. Effect of Age of Tissue. Plant Physiol. 1955 Jul;30(4):343–347.[PMC free article] [PubMed] [Google Scholar]
- Guerritore A, Hanozet GM, Cocucci MC. Regulation of isocitrate lyase level in yeast growing on external carbon sources or on lipid reserves. Experientia. 1969 Feb 15;25(2):131–132. [PubMed] [Google Scholar]
- HEYDEMAN MT. Isocitritase in germinating marrow seeds. Nature. 1958 Mar 1;181(4609):627–628. [PubMed] [Google Scholar]
- Johnson GV, Evans HJ, Ching T. Enzymes of the glyoxylate cycle in rhizobia and nodules of legumes. Plant Physiol. 1966 Oct;41(8):1330–1336.[PMC free article] [PubMed] [Google Scholar]
- Kornberg HL. The role and control of the glyoxylate cycle in Escherichia coli. Biochem J. 1966 Apr;99(1):1–11.[PMC free article] [PubMed] [Google Scholar]
- LEE HJ, KIM SJ, LEE KB. STUDY ON THE GLYOXYLATE CYCLE IN GERMINATING SESAME SEED EMBRYOS. Arch Biochem Biophys. 1964 Sep;107:479–484. [PubMed] [Google Scholar]
- Longo CP. Evidence for de novo synthesis of isocitratase and malate synthesis in germinating peanut cotyledons. Plant Physiol. 1968 Apr;43(4):660–664.[PMC free article] [PubMed] [Google Scholar]
- Rao GR, McFadden BA. Isocitrate lyase from Pseudomonas indigofera. IV. Specificity and inhibition. Arch Biochem Biophys. 1965 Nov;112(2):294–303. [PubMed] [Google Scholar]
- Weber G, Lea MA, Convery HJ, Stamm NB. Regulation of gluconeogenesis and glycolysis: studies of mechanisms controlling enzyme activity. Adv Enzyme Regul. 1967;5:257–300. [PubMed] [Google Scholar]