Two dehydrins from Agapanthus praecox (ApY₂SK₂ and ApSK₃) show important protective effects under complex stresses. Both ApY₂SK₂ and ApSK₃ contain one intron and consist of a full-length cDNA of 981 bp and 1057 bp encoding 186 and 215 amino acids, respectively. ApY₂SK₂ and ApSK₃ transgenic Arabidopsis thaliana show reduced plasma membrane damage and ROS levels and higher antioxidant activity and photosynthesis capability under salt, osmotic, cold and drought stresses compared with the wild-type. ApY₂SK₂ and ApSK₃ are mainly located in the cytoplasm and cell membrane, and ApY₂SK₂ can even localize in the nucleus. In vitro tests indicate that ApY₂SK₂ and ApSK₃ can effectively protect enzyme activity during the freeze-thaw process, and ApY₂SK₂ also exhibits this function during desiccation treatment. Furthermore, ApY₂SK₂ and ApSK₃ can significantly inhibit hydroxyl radical generation. These two dehydrins can bind metal ions with a binding affinity of Co²⁺> Ni²⁺> Cu²⁺> Fe³⁺; the binding affinity of ApSK₃ is higher than that of ApY₂SK₂. Thus, ApY₂SK₂ has a better protective effect on enzyme activity, and ApSK₃ has stronger metal ion binding function and effect on ROS metabolism. Moreover, plant cryopreservation evaluation tests indicate that ApY₂SK₂ and ApSK₃ transformation can enhance the seedling survival ratio from 23% to 47% and 55%, respectively; the addition of recombinant ApY₂SK₂ and ApSK₃ to plant vitrification solution may increase the survival ratio of wild-type A. thaliana seedlings from 24% to 50% and 46%, respectively. These findings suggest that ApY₂SK₂ and ApSK₃ can effectively improve cell stress tolerance and have great potential for in vivo or in vitro applications.