Bacterial-type phosphoenolpyruvate carboxylase (PEPC) functions as a catalytic and regulatory subunit of the novel class-2 PEPC complex of vascular plants.

Cloning, Transformation, and Heterologous Expression of Recombinant PEPCs

Full-length cDNAs encoding RcPpc3 and RcPpc4 were cloned as NdeI/XhoI and NdeI/NotI inserts, respectively, in a pET28b His tag vector (Novagen). The primer pairs used for RcPpc3 were NdeI-3 forward 5′-AGCCATATGCAACCAAGGAATTTAGAGATG-3′, and XhoI-3 reverse 5′-CTCGAGTTAACCAGTGTTTTGTAGTCCAGC-3′. Primers for RcPpc4 were NdeI-4 forward 5′-AGCCATATGACGGACACCACAGATGATATT-3′, and NotI-4 reverse 5′-GCGGCCGCTCAGCCTGTGTTCCTCATT-3′. The underlined nucleotide bases indicate restriction sites incorporated for directional cloning. Constructs used for producing recombinant His-tagged AtPPC3 and AtPPC4 were as previously described (11). PCR was performed with Pfu DNA polymerase (Fermentas) with full-length RcPpc3 and RcPpc4 cDNA as template, running 30 cycles of 95 °C for 30 s, 55 °C for 1 min, and 68 °C for 3 min. Both the vector and PCR product were digested with appropriate restriction enzymes, purified with a DNA gel extraction kit (MO BIO Laboratories), and directionally cloned overnight at room temperature with T4 DNA ligase (Fermentas). The ligated products were transformed in E. coli (BL21-CodonPlus (DE3)-RIL) (Stratagene) through electroporation and screened on LB plates containing 50 μg/ml kanamycin. The vector insert constructs were confirmed by restriction digestion of the isolated plasmid clones with Wizard Plus miniprep (Promega) and sequenced. Cells were cultured at 37 °C until an A₆₀₀ of 0.6 was reached, incubated for 5 min at 4 °C, then induced for 3 h at 20 °C with 0.1 mm isopropyl β-d-1-thiogalactopyranoside. Recombinant expression was according to Thomas and Baneyx (24) for RcPPC4 and Kim et al. (25) for AtPPC3. The cultures were centrifuged and pellets were quick frozen in liquid N₂ and stored at −80 °C.

Purification of Recombinant RcPPC4, AtPPC3, and Class-2 PEPC

All procedures were performed at 4 °C, except column chromatography, which was conducted using an ÄKTA Fast Protein Liquid Chromatography (FPLC) system (GE Healthcare) at room temperature (25 °C). Buffer A contained 50 mm NaH₂PO₄ (pH 8.0), 300 mm NaCl, 15% (v/v) glycerol, and 1 mm dithiothreitol. Buffer B contained 100 mm KH₂PO₄ (pH 8.0), 1 mm EDTA, 2 mm MgCl₂, 5 mm malate, 15% (v/v) glycerol, and 1 mm dithiothreitol. E. coli cells (16 g) containing recombinant RcPPC4 were thawed in 60 ml of buffer A and lysed by passage through a French press at 20,000 p.s.i. After centrifugation the supernatant was loaded at 1 ml/min onto a column (1.6 × 10 cm) of PrepEase^{His-tagged High Yield Purification Ni}^{-affinity resin (U. S. Biochemical Corp.). The column was washed with buffer A until the}A₂₈₀ approached baseline, then eluted with buffer A containing 150 mm imidazole. Pooled peak fractions were concentrated to 2 ml with an Amicon Ultra-15 centrifugal filter unit (100-kDa cutoff), then loaded at 0.3 ml/min onto a Superdex-200 HR 16/50 column equilibrated with buffer B. Pooled peak fractions were concentrated as above to <1 ml and stored at −20 °C in 50% (v/v) glycerol. The purified BTPC lost about 35% of its PEPC activity after 2 weeks of storage.

For simultaneous purification of recombinant Class-1 PEPC (AtPPC3) and chimeric Class-2 PEPC (containing recombinant RcPPC4 and AtPPC3), freshly prepared clarified extracts from 3 g of AtPPC3- and 6 g of RcPPC4-expressing E. coli cells were immediately mixed and subjected to PrepEase^Ni^{-affinity and Superdex-200 FPLC as described above. Pooled Class-1 and Class-2 PEPC peak fractions from the Superdex 200 column (}Fig. 1B) were separately concentrated as above to 250 μl and subjected to FPLC on a calibrated Superose-6 10/300 GL column (10) at 0.25 ml/min. Pooled peak fractions were concentrated as above to <1 ml and stored at −20 °C in 50% (v/v) glycerol. The PEPC activity of the final Class-1 and Class-2 PEPC preparations was stable for at least 2 months when stored under these conditions.

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Object name is zbc0410988600001.jpg

FIGURE 1.

Purification of recombinant RcPPC4, AtPPC3, and a chimeric Class-2 PEPC by gel filtration FPLC.A, Superdex-200 HR 16/50 elution profile for recombinant RcPPC4. B, Superdex-200 HR 16/50 elution profile indicating the position of Class-2 and Class-1 PEPCs obtained from a mixture of recombinant RcPPC4 and AtPPC3. Fractions eluting at 46–50 and 54–59 ml were pooled to enrich Class-2 and Class-1 PEPC, respectively. C and D, Superose-6 10/300 GL elution profiles of the chimeric Class-2 PEPC (RcPPC4 + AtPPC3) hetero-octamer (C) and Class-1 PEPC (AtPPC3) homotetramer (D). Insets, aliquots (A, 7-μl each; B–D, 2-μl each) from various fractions were subjected to SDS-PAGE followed by protein staining with Coomassie Brilliant Blue R-250 (CBB-R250) (A–D), or immunoblotting with anti-RcPPC4-IgG (A). V₀ denotes the void volume.

Electrophoresis and Immunoblotting

SDS and non-denaturing PAGE using a Bio-Rad Protean III mini-gel system, subunit and native M_r estimates via SDS- and non-denaturing PAGE, in-gel PEPC activity staining, and immunoblotting were as described previously (10). Antigenic polypeptides were visualized using an alkaline phosphatase-conjugated secondary antibody and chromogenic detection (26). All immunoblot results were replicated a minimum of three times with representative results shown in the figures. Rabbit anti-(native RcPPC3) IgG was raised against homogeneous Class-1 PEPC from developing COS as described previously (11). Rabbit antiserum was also raised against homogeneous recombinant RcPPC4 that had been dialyzed overnight in P_i-buffered saline and emulsified 1:1 in TiterMax Gold adjuvant (CytRx Corp.). Following collection of preimmune serum, 200 μg of RcPPC4 was injected subcutaneously into a rabbit, and a 150-μg booster injection was administered 28 days later. At 9 days after the final injection, blood was collected in Vacutainer tubes (Becton Dickinson) by cardiac puncture. Clotted cells were removed by centrifugation and the immune serum frozen in liquid N₂ and stored at −80 °C in 0.04% (w/v) NaN₃. For immunoblotting, anti-RcPPC4-IgG was affinity purified against 500 μg of nitrocellulose-bound homogeneous recombinant RcPPC4 as previously described (26).

Co-immunopurification

This was performed as previously described (14). A clarified extract from 5 g of stage V (mid-cotyledon) developing COS endosperm was eluted at 0.5 ml/min through a column containing 1.5 mg of protein A-purified anti-RcPPC4-IgG that had been covalently coupled to 1 ml of AminoLink plus gel (Pierce Chemicals) as per the manufacturer's instructions. After washing non-bound proteins from the column with P_i-buffered saline containing 10 μl/ml ProteCEASE-100 (G-Biosciences), absorbed proteins were eluted with 100 mm glycine HCl (pH 2.8), neutralized with unbuffered Tris, and concentrated to approximately 1 mg/ml prior to analysis of their polypeptide composition by SDS-PAGE and immunoblotting.

Enzyme and Protein Assays and Kinetic Studies

The PEPC activity was assayed at 25 °C by following NADH oxidation at 340 nm in a final volume of 200 μl using a Spectramax Plus microplate reader (Molecular Devices) and the following optimized assay mixture: 50 mm Hepes-KOH (pH 8.0) containing 10% (v/v) glycerol, 10 mm PEP, 5 mm KHCO₃, 10 mm MgCl₂, 2 mm dithiothreitol, 0.15 mm NADH, and 5 units/ml of desalted porcine muscle malate dehydrogenase. One unit of PEPC is defined as the amount of enzyme resulting in the production of 1 μmol of oxaloacetate/min. Protein concentrations were determined by the Coomassie Blue G-250 dye binding method using bovine γ-globulin as the protein standard (10). Apparent V_max and K_m_(PEP), and IC₅₀ values (inhibitor concentration producing 50% inhibition of PEPC activity) were routinely calculated using a nonlinear least square regression computer program (27). For the chimeric Class-2 PEPC, PEP saturation data were fitted to both single and two active site models using nonlinear regression analysis software (SigmaPlot Version 10.0 (SPSS Inc.)) as previously described (17). All kinetic parameters represent means of at least four separate determinations and are reproducible to within ±15% (S.E.) of the mean value unless otherwise noted. Stock solutions of all metabolites were made equimolar with MgCl₂ and adjusted to pH 7.0.

Statistics

Data were analyzed using the Student's t test, and deemed significant at p < 0.05.

Cloning, Transformation, and Heterologous Expression of Recombinant PEPCs

Full-length cDNAs encoding RcPpc3 and RcPpc4 were cloned as NdeI/XhoI and NdeI/NotI inserts, respectively, in a pET28b His tag vector (Novagen). The primer pairs used for RcPpc3 were NdeI-3 forward 5′-AGCCATATGCAACCAAGGAATTTAGAGATG-3′, and XhoI-3 reverse 5′-CTCGAGTTAACCAGTGTTTTGTAGTCCAGC-3′. Primers for RcPpc4 were NdeI-4 forward 5′-AGCCATATGACGGACACCACAGATGATATT-3′, and NotI-4 reverse 5′-GCGGCCGCTCAGCCTGTGTTCCTCATT-3′. The underlined nucleotide bases indicate restriction sites incorporated for directional cloning. Constructs used for producing recombinant His-tagged AtPPC3 and AtPPC4 were as previously described (11). PCR was performed with Pfu DNA polymerase (Fermentas) with full-length RcPpc3 and RcPpc4 cDNA as template, running 30 cycles of 95 °C for 30 s, 55 °C for 1 min, and 68 °C for 3 min. Both the vector and PCR product were digested with appropriate restriction enzymes, purified with a DNA gel extraction kit (MO BIO Laboratories), and directionally cloned overnight at room temperature with T4 DNA ligase (Fermentas). The ligated products were transformed in E. coli (BL21-CodonPlus (DE3)-RIL) (Stratagene) through electroporation and screened on LB plates containing 50 μg/ml kanamycin. The vector insert constructs were confirmed by restriction digestion of the isolated plasmid clones with Wizard Plus miniprep (Promega) and sequenced. Cells were cultured at 37 °C until an A₆₀₀ of 0.6 was reached, incubated for 5 min at 4 °C, then induced for 3 h at 20 °C with 0.1 mm isopropyl β-d-1-thiogalactopyranoside. Recombinant expression was according to Thomas and Baneyx (24) for RcPPC4 and Kim et al. (25) for AtPPC3. The cultures were centrifuged and pellets were quick frozen in liquid N₂ and stored at −80 °C.

Purification of Recombinant RcPPC4, AtPPC3, and Class-2 PEPC

All procedures were performed at 4 °C, except column chromatography, which was conducted using an ÄKTA Fast Protein Liquid Chromatography (FPLC) system (GE Healthcare) at room temperature (25 °C). Buffer A contained 50 mm NaH₂PO₄ (pH 8.0), 300 mm NaCl, 15% (v/v) glycerol, and 1 mm dithiothreitol. Buffer B contained 100 mm KH₂PO₄ (pH 8.0), 1 mm EDTA, 2 mm MgCl₂, 5 mm malate, 15% (v/v) glycerol, and 1 mm dithiothreitol. E. coli cells (16 g) containing recombinant RcPPC4 were thawed in 60 ml of buffer A and lysed by passage through a French press at 20,000 p.s.i. After centrifugation the supernatant was loaded at 1 ml/min onto a column (1.6 × 10 cm) of PrepEase^{His-tagged High Yield Purification Ni}^{-affinity resin (U. S. Biochemical Corp.). The column was washed with buffer A until the}A₂₈₀ approached baseline, then eluted with buffer A containing 150 mm imidazole. Pooled peak fractions were concentrated to 2 ml with an Amicon Ultra-15 centrifugal filter unit (100-kDa cutoff), then loaded at 0.3 ml/min onto a Superdex-200 HR 16/50 column equilibrated with buffer B. Pooled peak fractions were concentrated as above to <1 ml and stored at −20 °C in 50% (v/v) glycerol. The purified BTPC lost about 35% of its PEPC activity after 2 weeks of storage.

For simultaneous purification of recombinant Class-1 PEPC (AtPPC3) and chimeric Class-2 PEPC (containing recombinant RcPPC4 and AtPPC3), freshly prepared clarified extracts from 3 g of AtPPC3- and 6 g of RcPPC4-expressing E. coli cells were immediately mixed and subjected to PrepEase^Ni^{-affinity and Superdex-200 FPLC as described above. Pooled Class-1 and Class-2 PEPC peak fractions from the Superdex 200 column (}Fig. 1B) were separately concentrated as above to 250 μl and subjected to FPLC on a calibrated Superose-6 10/300 GL column (10) at 0.25 ml/min. Pooled peak fractions were concentrated as above to <1 ml and stored at −20 °C in 50% (v/v) glycerol. The PEPC activity of the final Class-1 and Class-2 PEPC preparations was stable for at least 2 months when stored under these conditions.

FIGURE 1.

Purification of recombinant RcPPC4, AtPPC3, and a chimeric Class-2 PEPC by gel filtration FPLC.A, Superdex-200 HR 16/50 elution profile for recombinant RcPPC4. B, Superdex-200 HR 16/50 elution profile indicating the position of Class-2 and Class-1 PEPCs obtained from a mixture of recombinant RcPPC4 and AtPPC3. Fractions eluting at 46–50 and 54–59 ml were pooled to enrich Class-2 and Class-1 PEPC, respectively. C and D, Superose-6 10/300 GL elution profiles of the chimeric Class-2 PEPC (RcPPC4 + AtPPC3) hetero-octamer (C) and Class-1 PEPC (AtPPC3) homotetramer (D). Insets, aliquots (A, 7-μl each; B–D, 2-μl each) from various fractions were subjected to SDS-PAGE followed by protein staining with Coomassie Brilliant Blue R-250 (CBB-R250) (A–D), or immunoblotting with anti-RcPPC4-IgG (A). V₀ denotes the void volume.

Electrophoresis and Immunoblotting

SDS and non-denaturing PAGE using a Bio-Rad Protean III mini-gel system, subunit and native M_r estimates via SDS- and non-denaturing PAGE, in-gel PEPC activity staining, and immunoblotting were as described previously (10). Antigenic polypeptides were visualized using an alkaline phosphatase-conjugated secondary antibody and chromogenic detection (26). All immunoblot results were replicated a minimum of three times with representative results shown in the figures. Rabbit anti-(native RcPPC3) IgG was raised against homogeneous Class-1 PEPC from developing COS as described previously (11). Rabbit antiserum was also raised against homogeneous recombinant RcPPC4 that had been dialyzed overnight in P_i-buffered saline and emulsified 1:1 in TiterMax Gold adjuvant (CytRx Corp.). Following collection of preimmune serum, 200 μg of RcPPC4 was injected subcutaneously into a rabbit, and a 150-μg booster injection was administered 28 days later. At 9 days after the final injection, blood was collected in Vacutainer tubes (Becton Dickinson) by cardiac puncture. Clotted cells were removed by centrifugation and the immune serum frozen in liquid N₂ and stored at −80 °C in 0.04% (w/v) NaN₃. For immunoblotting, anti-RcPPC4-IgG was affinity purified against 500 μg of nitrocellulose-bound homogeneous recombinant RcPPC4 as previously described (26).

Co-immunopurification

This was performed as previously described (14). A clarified extract from 5 g of stage V (mid-cotyledon) developing COS endosperm was eluted at 0.5 ml/min through a column containing 1.5 mg of protein A-purified anti-RcPPC4-IgG that had been covalently coupled to 1 ml of AminoLink plus gel (Pierce Chemicals) as per the manufacturer's instructions. After washing non-bound proteins from the column with P_i-buffered saline containing 10 μl/ml ProteCEASE-100 (G-Biosciences), absorbed proteins were eluted with 100 mm glycine HCl (pH 2.8), neutralized with unbuffered Tris, and concentrated to approximately 1 mg/ml prior to analysis of their polypeptide composition by SDS-PAGE and immunoblotting.

Enzyme and Protein Assays and Kinetic Studies

The PEPC activity was assayed at 25 °C by following NADH oxidation at 340 nm in a final volume of 200 μl using a Spectramax Plus microplate reader (Molecular Devices) and the following optimized assay mixture: 50 mm Hepes-KOH (pH 8.0) containing 10% (v/v) glycerol, 10 mm PEP, 5 mm KHCO₃, 10 mm MgCl₂, 2 mm dithiothreitol, 0.15 mm NADH, and 5 units/ml of desalted porcine muscle malate dehydrogenase. One unit of PEPC is defined as the amount of enzyme resulting in the production of 1 μmol of oxaloacetate/min. Protein concentrations were determined by the Coomassie Blue G-250 dye binding method using bovine γ-globulin as the protein standard (10). Apparent V_max and K_m_(PEP), and IC₅₀ values (inhibitor concentration producing 50% inhibition of PEPC activity) were routinely calculated using a nonlinear least square regression computer program (27). For the chimeric Class-2 PEPC, PEP saturation data were fitted to both single and two active site models using nonlinear regression analysis software (SigmaPlot Version 10.0 (SPSS Inc.)) as previously described (17). All kinetic parameters represent means of at least four separate determinations and are reproducible to within ±15% (S.E.) of the mean value unless otherwise noted. Stock solutions of all metabolites were made equimolar with MgCl₂ and adjusted to pH 7.0.

Statistics

Data were analyzed using the Student's t test, and deemed significant at p < 0.05.

Cloning, Transformation, and Heterologous Expression of Recombinant PEPCs

Full-length cDNAs encoding RcPpc3 and RcPpc4 were cloned as NdeI/XhoI and NdeI/NotI inserts, respectively, in a pET28b His tag vector (Novagen). The primer pairs used for RcPpc3 were NdeI-3 forward 5′-AGCCATATGCAACCAAGGAATTTAGAGATG-3′, and XhoI-3 reverse 5′-CTCGAGTTAACCAGTGTTTTGTAGTCCAGC-3′. Primers for RcPpc4 were NdeI-4 forward 5′-AGCCATATGACGGACACCACAGATGATATT-3′, and NotI-4 reverse 5′-GCGGCCGCTCAGCCTGTGTTCCTCATT-3′. The underlined nucleotide bases indicate restriction sites incorporated for directional cloning. Constructs used for producing recombinant His-tagged AtPPC3 and AtPPC4 were as previously described (11). PCR was performed with Pfu DNA polymerase (Fermentas) with full-length RcPpc3 and RcPpc4 cDNA as template, running 30 cycles of 95 °C for 30 s, 55 °C for 1 min, and 68 °C for 3 min. Both the vector and PCR product were digested with appropriate restriction enzymes, purified with a DNA gel extraction kit (MO BIO Laboratories), and directionally cloned overnight at room temperature with T4 DNA ligase (Fermentas). The ligated products were transformed in E. coli (BL21-CodonPlus (DE3)-RIL) (Stratagene) through electroporation and screened on LB plates containing 50 μg/ml kanamycin. The vector insert constructs were confirmed by restriction digestion of the isolated plasmid clones with Wizard Plus miniprep (Promega) and sequenced. Cells were cultured at 37 °C until an A₆₀₀ of 0.6 was reached, incubated for 5 min at 4 °C, then induced for 3 h at 20 °C with 0.1 mm isopropyl β-d-1-thiogalactopyranoside. Recombinant expression was according to Thomas and Baneyx (24) for RcPPC4 and Kim et al. (25) for AtPPC3. The cultures were centrifuged and pellets were quick frozen in liquid N₂ and stored at −80 °C.

Purification of Recombinant RcPPC4, AtPPC3, and Class-2 PEPC

All procedures were performed at 4 °C, except column chromatography, which was conducted using an ÄKTA Fast Protein Liquid Chromatography (FPLC) system (GE Healthcare) at room temperature (25 °C). Buffer A contained 50 mm NaH₂PO₄ (pH 8.0), 300 mm NaCl, 15% (v/v) glycerol, and 1 mm dithiothreitol. Buffer B contained 100 mm KH₂PO₄ (pH 8.0), 1 mm EDTA, 2 mm MgCl₂, 5 mm malate, 15% (v/v) glycerol, and 1 mm dithiothreitol. E. coli cells (16 g) containing recombinant RcPPC4 were thawed in 60 ml of buffer A and lysed by passage through a French press at 20,000 p.s.i. After centrifugation the supernatant was loaded at 1 ml/min onto a column (1.6 × 10 cm) of PrepEase^{His-tagged High Yield Purification Ni}^{-affinity resin (U. S. Biochemical Corp.). The column was washed with buffer A until the}A₂₈₀ approached baseline, then eluted with buffer A containing 150 mm imidazole. Pooled peak fractions were concentrated to 2 ml with an Amicon Ultra-15 centrifugal filter unit (100-kDa cutoff), then loaded at 0.3 ml/min onto a Superdex-200 HR 16/50 column equilibrated with buffer B. Pooled peak fractions were concentrated as above to <1 ml and stored at −20 °C in 50% (v/v) glycerol. The purified BTPC lost about 35% of its PEPC activity after 2 weeks of storage.

For simultaneous purification of recombinant Class-1 PEPC (AtPPC3) and chimeric Class-2 PEPC (containing recombinant RcPPC4 and AtPPC3), freshly prepared clarified extracts from 3 g of AtPPC3- and 6 g of RcPPC4-expressing E. coli cells were immediately mixed and subjected to PrepEase^Ni^{-affinity and Superdex-200 FPLC as described above. Pooled Class-1 and Class-2 PEPC peak fractions from the Superdex 200 column (}Fig. 1B) were separately concentrated as above to 250 μl and subjected to FPLC on a calibrated Superose-6 10/300 GL column (10) at 0.25 ml/min. Pooled peak fractions were concentrated as above to <1 ml and stored at −20 °C in 50% (v/v) glycerol. The PEPC activity of the final Class-1 and Class-2 PEPC preparations was stable for at least 2 months when stored under these conditions.

FIGURE 1.

Purification of recombinant RcPPC4, AtPPC3, and a chimeric Class-2 PEPC by gel filtration FPLC.A, Superdex-200 HR 16/50 elution profile for recombinant RcPPC4. B, Superdex-200 HR 16/50 elution profile indicating the position of Class-2 and Class-1 PEPCs obtained from a mixture of recombinant RcPPC4 and AtPPC3. Fractions eluting at 46–50 and 54–59 ml were pooled to enrich Class-2 and Class-1 PEPC, respectively. C and D, Superose-6 10/300 GL elution profiles of the chimeric Class-2 PEPC (RcPPC4 + AtPPC3) hetero-octamer (C) and Class-1 PEPC (AtPPC3) homotetramer (D). Insets, aliquots (A, 7-μl each; B–D, 2-μl each) from various fractions were subjected to SDS-PAGE followed by protein staining with Coomassie Brilliant Blue R-250 (CBB-R250) (A–D), or immunoblotting with anti-RcPPC4-IgG (A). V₀ denotes the void volume.

Electrophoresis and Immunoblotting

SDS and non-denaturing PAGE using a Bio-Rad Protean III mini-gel system, subunit and native M_r estimates via SDS- and non-denaturing PAGE, in-gel PEPC activity staining, and immunoblotting were as described previously (10). Antigenic polypeptides were visualized using an alkaline phosphatase-conjugated secondary antibody and chromogenic detection (26). All immunoblot results were replicated a minimum of three times with representative results shown in the figures. Rabbit anti-(native RcPPC3) IgG was raised against homogeneous Class-1 PEPC from developing COS as described previously (11). Rabbit antiserum was also raised against homogeneous recombinant RcPPC4 that had been dialyzed overnight in P_i-buffered saline and emulsified 1:1 in TiterMax Gold adjuvant (CytRx Corp.). Following collection of preimmune serum, 200 μg of RcPPC4 was injected subcutaneously into a rabbit, and a 150-μg booster injection was administered 28 days later. At 9 days after the final injection, blood was collected in Vacutainer tubes (Becton Dickinson) by cardiac puncture. Clotted cells were removed by centrifugation and the immune serum frozen in liquid N₂ and stored at −80 °C in 0.04% (w/v) NaN₃. For immunoblotting, anti-RcPPC4-IgG was affinity purified against 500 μg of nitrocellulose-bound homogeneous recombinant RcPPC4 as previously described (26).

Co-immunopurification

This was performed as previously described (14). A clarified extract from 5 g of stage V (mid-cotyledon) developing COS endosperm was eluted at 0.5 ml/min through a column containing 1.5 mg of protein A-purified anti-RcPPC4-IgG that had been covalently coupled to 1 ml of AminoLink plus gel (Pierce Chemicals) as per the manufacturer's instructions. After washing non-bound proteins from the column with P_i-buffered saline containing 10 μl/ml ProteCEASE-100 (G-Biosciences), absorbed proteins were eluted with 100 mm glycine HCl (pH 2.8), neutralized with unbuffered Tris, and concentrated to approximately 1 mg/ml prior to analysis of their polypeptide composition by SDS-PAGE and immunoblotting.

Enzyme and Protein Assays and Kinetic Studies

The PEPC activity was assayed at 25 °C by following NADH oxidation at 340 nm in a final volume of 200 μl using a Spectramax Plus microplate reader (Molecular Devices) and the following optimized assay mixture: 50 mm Hepes-KOH (pH 8.0) containing 10% (v/v) glycerol, 10 mm PEP, 5 mm KHCO₃, 10 mm MgCl₂, 2 mm dithiothreitol, 0.15 mm NADH, and 5 units/ml of desalted porcine muscle malate dehydrogenase. One unit of PEPC is defined as the amount of enzyme resulting in the production of 1 μmol of oxaloacetate/min. Protein concentrations were determined by the Coomassie Blue G-250 dye binding method using bovine γ-globulin as the protein standard (10). Apparent V_max and K_m_(PEP), and IC₅₀ values (inhibitor concentration producing 50% inhibition of PEPC activity) were routinely calculated using a nonlinear least square regression computer program (27). For the chimeric Class-2 PEPC, PEP saturation data were fitted to both single and two active site models using nonlinear regression analysis software (SigmaPlot Version 10.0 (SPSS Inc.)) as previously described (17). All kinetic parameters represent means of at least four separate determinations and are reproducible to within ±15% (S.E.) of the mean value unless otherwise noted. Stock solutions of all metabolites were made equimolar with MgCl₂ and adjusted to pH 7.0.

Statistics

Data were analyzed using the Student's t test, and deemed significant at p < 0.05.

Heterologous Expression, Purification, and Immunological Characterization of the Bacterial-type PEP Carboxylase RcPPC4 from Developing Castor Oil Seeds

Full-length RcPPC4-encoding cDNA (11) was subcloned into a pET28b expression vector with an N-terminal His₆ tag and transformed into several expression strains. Optimal results were obtained with E. coli BL21(DE3) in which RcPPC4 was expressed as an active PEPC in the soluble fraction. About 1 mg of RcPPC4 was purified 250-fold to apparent homogeneity and a final specific PEPC activity of 27 units/mg by a combination of Ni^{affinity and Superdex-200 FPLC (}Figs. 1A and and22A, supplemental Table S1). The tendency of RcPPC4 to form large aggregates was reflected by its consistent elution in the void volume during Superdex-200 or Superose-6 gel filtration FPLC (molecular mass > 2 MDa), and efficient precipitation with a relatively low concentration (5%, w/v) of polyethylene glycol 8,000 (Fig. 1A and results not shown). The final RcPPC4 preparation proved difficult to store as it slowly lost activity when maintained at −20 °C in 50% (v/v) glycerol, and was completely inactivated when rapidly thawed after freezing in liquid N₂. After digestion with the endopeptidase thrombin to remove the His₆ tag used for purification, RcPPC4 was cleaved into approximately 64- and 50-kDa polypeptides (supplemental Fig. S2). This parallels the well documented susceptibility of the native RcPPC4 to rapid proteolysis to similarly sized polypeptides by an endogenous thiol endopeptidase during incubation of clarified COS extracts on ice, or native Class-2 PEPC purification from developing COS (supplemental Fig. S2) (10, 11, 13). Thus, all subsequent studies were conducted using His₆-tagged RcPPC4.

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Object name is zbc0410988600002.jpg

FIGURE 2.

SDS-PAGE and immunoblot analysis of purified recombinant PEPC isoforms.A, SDS-PAGE followed by Coomassie Brilliant Blue (CBB) R-250 staining was performed on various fractions obtained during the purification of the recombinant BTPC, RcPPC4. Lanes 1–4, respectively, contain 20, 20, 10, and 2.5 μg of the clarified soluble extract, corresponding insoluble (inclusion body) fraction, pooled PrepEase^Ni^{-affinity fractions, and pooled Superdex-200 fractions.}B, various amounts of a clarified extract from stage V (mid-cotyledon) developing COS and purified recombinant RcPPC4 were subjected to SDS-PAGE followed by immunoblotting with a 1:250 dilution of affinity purified anti-RcPPC4-IgG. Lanes 1–3, respectively, contain 10, 5, and 2.5 μg of the clarified COS extract, whereas lanes 4–6 contain 25, 10, and 2.5 ng of pure RcPPC4. C-E, aliquots from various stages of concurrent Class-2 PEPC (RcPPC4 + AtPPC3) and Class-1 PEPC (AtPPC3) purification were subjected to SDS-PAGE followed by staining with Coomassie Brilliant Blue R-250 (C), and immunoblotting with anti-RcPPC4-IgG (D) or anti-RcPPC3-IgG (E). Lane 1 of panels C–E, respectively, correspond to 60, 0.2, and 3 μg of the combined clarified E. coli extracts containing soluble recombinant AtPPC3 and RcPPC4. Likewise, lanes 2 of panels C–E, respectively, contain 5, 0.3, and 1.5 μg of the pooled Ni^{-affinity column fractions;}lanes 3 and 4 of panels C–E, respectively, contain 2, 0.1, and 0.02 μg of pooled chimeric Class-2 PEPC fractions from the Superdex-200 (lane 3) and Superose-6 (lane 4) columns; lanes 5 and 6, respectively, contain 2, 0.1, and 0.01 μg of the pooled Class-1 PEPC AtPPC3 fractions from the Superdex-200 (lane 5) and Superose-6 (lane 6) columns. M denotes various prestained SDS-PAGE M_r standard.

Rabbit immunization with purified RcPPC4 led to the production of affinity purified anti-RcPPC4-IgG that cross-reacted with as little as 10 ng of the corresponding antigen and was monospecific for 118-kDa RcPPC4 polypeptides on immunoblots of developing COS extracts (Fig. 2B). By contrast, immunoblots of RcPPC4 failed to cross-react with anti-RcPPC3-IgG and vice versa (Fig. 2, B, D, and E). This corroborates the well documented sequence and immunological distinctiveness between vascular plant and green algal BTPCs and PTPCs (8, 10, 11, 15, 17, 19, 20). Nevertheless, RcPPC4 quantitatively co-immunopurified with a similar amount of RcPPC3 following elution of developing COS extracts through an anti-RcPPC4-IgG immunoaffinity column (Fig. 3A). Non-denaturing PAGE followed by in-gel PEPC activity staining and parallel spectrophotometric PEPC activity assays established that 100% of Class-2 PEPC corresponding to 48 ± 6% (mean ± S.E., n = 3) of the total PEPC activity present in the initial clarified COS extract was absorbed by the anti-RcPPC4-IgG co-immunopurification (co-IP) column (Fig. 3B). By contrast, the Class-1 PEPC (RcPPC3) homotetramer was eluted in the corresponding flow-through/unbound fractions.

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FIGURE 3.

Co-immunopurification of the native Class-2 PEPC complex from developing COS endosperm by anti-RcPPC4-IgG immunoaffinity chromatography. A clarified extract from 5 g of stage V (mid-cotyledon) developing COS endosperm was precleared with a preimmune serum protein column (14) prior to elution through the anti-RcPPC4-IgG column. A, bound proteins eluted from the anti-RcPPC4-IgG column with Gly-HCl (pH 2.8) (co-IP) and pooled unbound flow-through fractions (Co-IP FT) were analyzed by 10% SDS-PAGE followed by Coomassie Brilliant Blue (CBB) R-250 staining or immunoblotting with anti-(RcPPC3 or RcPPC4)-IgG. Co-IP and co-IP flow-through lanes, respectively, contained 8 and 40 μg of protein (for Coomassie Brilliant Blue R-250 staining) or 1 and 20 μg of protein (for immunoblotting). B, the PEPC isoforms present in the initial clarified extract (Extract) and pooled co-IP flow-through fractions were visualized by 5% non-denaturing PAGE followed by in-gel PEPC activity staining (3 milliunits/lane).

Recombinant RcPPC4 Forms Class-2 PEPC When Combined with Class-1 PEPCs

Non-denaturing PAGE of recombinant RcPPC4 failed to produce a PEPC activity staining band, but generated an anti-RcPPC4-IgG immunoreactive smear that reflects its propensity to aggregate into insoluble precipitates (Fig. 4, A and B). However, when preincubated with homogenous native Class-1 PEPC from developing COS and analyzed by non-denaturing PAGE, recombinant RcPPC4 produced a Class-2 PEPC activity staining band composed of immunoreactive RcPPC3 and RcPPC4 polypeptides that co-migrated with the native 910-kDa Class-2 PEPC from stage III developing COS (Fig. 4, A–C). Above a 1:1 stoichiometric ratio of recombinant RcPPC4:native RcPPC3, only modest gains in the proportion of Class-2 PEPC relative to Class-1 PEPC were obtained, with the bulk of RcPPC4 visualized as an immunoreactive smear (Fig. 4, A and B). Non-denaturing PAGE followed by in-gel PEPC activity staining also established that RcPPC4 was associated into Class-2 PEPC complexes when preincubated with purified native Class-1 PEPCs from germinating COS (5), Arabidopsis and Brassica napus suspension cell cultures (28, 29), and ripened banana fruit (30) (Fig. 4D). Slower migrating, higher M_r PEPC isoforms were also evident on the non-denaturing gels when the recombinant RcPPC4 was mixed with several Class-1 PEPCs (Fig. 4D). This is reminiscent of native Class-2 PEPC isoforms from the green alga Selenastrum minutum, which exists as three large but kinetically similar protein complexes containing various stoichiometric ratios of BTPC and PTPC polypeptides (15, 17, 18).

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FIGURE 4.

Non-denaturing PAGE analysis of in vitro Class-2 PEPC formation.A–D, homogeneous native Class-1 PEPCs were incubated with the purified recombinant RcPPC4 for 1 h at 25 °C in 50 mm Hepes-KOH, pH 7.7 (final volume = 25-μl). The mixtures were subjected to non-denaturing PAGE (7% separating gel) and in-gel PEPC activity staining (A and D) or immunoblotting with anti-RcPPC4-IgG (B) or anti-RcPPC3-IgG (C). A–C, various amounts of RcPPC4 were incubated with homogeneous Class-1 PEPC from developing COS (RcPPC3; 5.5 μg). Lanes 1–6 contain 2.5 μg of RcPPC3 and a proportional amount of RcPPC4; lane 7 contains 5 μg of RcPPC4; lane 8 contains 20 μg of clarified extract from stage III (heart-shaped embryo) COS, wherein Class-2 PEPC is the predominant isoform (10). D, purified native Class-1 PEPCs from various vascular plant sources (10 milliunits each) were incubated with (+) or without (−) 10 milliunits of purified RcPPC4 as described above. The designations are as follows: Banana, banana fruit PEPC (30); B. napus, PEPC from P_i-starved B. napus suspension cell cultures (28); Arabidopsis, AtPPC1 from P_i-starved Arabidopsis suspension cells (29); Germ. COS, monoubiquitinated Class-1 PEPC (RcPPC3) from germinating COS (5). Dev. COS denotes 10 milliunits of PEPC activity from a clarified extract prepared from stage V (mid-cotyledon) developing COS endosperm. E, non-denaturing PAGE followed by in-gel PEPC activity staining was performed on purified recombinant AtPPC3 and chimeric Class-2 PEPC together with a clarified extract from stage V developing COS (4 milliunits/lane).

Purification of a Non-proteolyzed Chimeric Class-2 PEPC from Recombinant RcPPC4 and AtPPC3

Heterologous expression of recombinant PEPCs in E. coli BL21(DE3) was also attempted with RcPPC3 and AtPPC4 (Arabidopsis BTPC ortholog; 79% identity). Both failed to yield soluble, active PEPC under a variety of induction conditions using constructs with and without an N-terminal His₆ tag. Similarly, co-expression of either AtPPC3 with AtPPC4, or RcPPC3 with RcPPC4 using the pETDuet-1 vector system (Novagen) did not result in active Class-2 PEPCs (results not shown). However, AtPPC3 (Arabidopsis PTPC ortholog of RcPPC3; 89% identity) was readily expressed as an active, soluble Class-1 PEPC composed of 107-kDa subunits as previously described (11). Freshly prepared, clarified extracts from E. coli expressing RcPPC4 and AtPPC3 were mixed at 25 °C and 2.4 mg of a chimeric Class-2 PEPC complex subsequently purified about 150-fold to apparent homogeneity by a combination of Ni^{affinity, and sequential Superdex-200 and Superose-6 gel filtration FPLC (}Figs. 1, B and C, and and2,2, C-E, Table 1). The Class-2 PEPC activity peak obtained during Superdex-200 or Superose-6 FPLC co-eluted with a 1:1 ratio of protein-staining RcPPC4 and AtPPC3 subunits (Fig. 1, B and C). The native M_r of the Class-2 PEPC as estimated by FPLC on a calibrated Superose-6 column was 900 ± 15 kDa (mean ± S.E., n = 3) (Fig. 1C), which corroborates its co-migration with the 910-kDa native Class-2 PEPC from stage III developing COS during non-denaturing PAGE (Fig. 4E). Thus, the purified chimeric Class-2 PEPC exists as a hetero-octamer composed of an equivalent ratio of non-proteolyzed 118-kDa RcPPC4 and 107-kDa AtPPC3 subunits. Class-1 PEPC was not detected in the final Class-2 PEPC preparation (Fig. 4E). Moreover: (i) the Class-2 PEPC complex did not dissociate over time as no Class-1 PEPC was visualized in subsequent PEPC activity stained non-denaturing gels (results not shown), and (ii) throughout several purification trials the RcPPC4 subunits were quantitatively bound by AtPPC3 to form the hetero-octameric Class-2 PEPC (Fig. 1, B and C). About 4 mg of excess AtPPC3 was simultaneously purified to a final specific activity of 15.1 units/mg (Figs. 1, B and D, and and2,2, C and E, Table 1). The native M_r of AtPPC3 as estimated during FPLC on the calibrated Superose-6 column was 440 ± 5 kDa (mean ± S.E., n = 3) (Fig. 1D), indicating that it exists as a typical Class-1 PEPC homotetramer composed of identical 107-kDa subunits. However, AtPPC3 appeared to dissociate into active dimers upon non-denaturing PAGE (Fig. 4E).

TABLE 1

Purification of recombinant Class-1 and -2 PEPCs from combined clarified extracts originating from 3 g of AtPPC3- and 6 g of RcPPC4-expressing E. coli cells

Step	Activity	Protein	Specific activity	Purification	Yield
	units	mg	units/mg	-fold	%
Clarified extracts
AtPPC3	164	540	0.30
RcPPC4	88	1458	0.060
Combined extracts	270	2140	0.13	1	100
Ni^{-affinity FPLC}	256	60	4.3	34	95
Superdex-200 FPLC
Class-1 PEPC	71.3	12.9	5.5	44	26
Class-2 PEPC	87.7	7.8	11.2	89	32
Superose-6 FPLC
Class-1 PEPC	66.6	4.4	15.1	106	25
Class-2 PEPC	60.5	2.4	25.2	152	22

The three recombinant PEPC isoforms differed in their thermal stability (Fig. 5). Similar to Class-1 PEPC homotetramers from developing COS and green algae (10, 15 –17), purified AtPPC3 was relatively heat-labile, losing over 95% of its activity when preincubated at 50 °C for 3 min. By contrast, the RcPPC4 was comparatively heat-stable, whereas the chimeric Class-2 PEPC exhibited an intermediate thermal stability (Fig. 5). This is consistent with previous studies documenting the enhanced thermal stability of green algal and vascular plant Class-2 PEPCs, relative to the corresponding Class-1 PEPC isoform (10, 15 –17).

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FIGURE 5.

Heat denaturation profiles of recombinant PEPCs. Aliquots of purified PEPCs were incubated at a given temperature for 3 min. The samples were cooled on ice for 3 min, and residual PEPC activity determined at 25 °C. Activities are expressed relative to the corresponding control set at 100% (equivalent to 15, 27, and 25 units/mg of AtPPC3, RcPPC4, and chimeric Class-2 PEPC, respectively). All values represent the means of three different experiments and are reproducible to within ±10% of the mean value.

Kinetic Properties

Effect of pH and Metal Cofactor Requirements

Similar to other plant PEPCs (1), the activity of the purified recombinant AtPPC3, RcPPC4, and chimeric Class-2 PEPC: (i) displayed a broad pH profile with optimal activity occurring in the pH 8.0–8.5 range (supplemental Fig. S3), and (ii) was absolutely dependent upon the presence of a bivalent metal cation cofactor, specifically Mg^{or Mn}^{. Metal ion requirements of RcPPC4 were investigated in more detail. It exhibited a 4-fold higher specific activity with saturating (10 m}m) Mg^{relative to Mn}^{, and a}K_m(Mg⁾ value of 0.10 ± 0.01 mm.

PEP Saturation Kinetics

RcPPC4 and AtPPC3 both displayed hyperbolic PEP saturation kinetics at pH 8.0 and physiological pH (7.3) (Table 2). However, the apparent K_m_(PEP) value of RcPPC4 at both pH values was about an order of magnitude greater than that of AtPPC3 whose K_m_(PEP) of approximately 70 μm is typical for a C₃ plant PEPC (1). PEP binding to the chimeric Class-2 PEPC was analyzed by fitting PEP saturation data to a single catalytic site Michaelis-Menten model or to a two-catalytic site Michaelian model using non-linear regression. The fit to the two-catalytic site model yielded a higher probability (p < 0.001). The biphasic PEP saturation kinetics of the Class-2 PEPC was visualized by Eadie-Hofstee plots of the kinetic data (Fig. 6A). The theoretical plot of the two-site model (solid line) fit the experimental points with a correlation coefficient of 0.99, whereas the plot obtained assuming a single catalytic site (dashed line) has a far lower correlation coefficient of 0.87. These results indicate that the RcPPC4 and AtPPC3 subunits are both catalytically active within the Class-2 PEPC, as previously inferred for a green algal Class-2 PEPC (17). The apparent K_m_(PEP) values for the two sites differed by approximately 15-fold with the high K_m site (attributed to RcPPC4) displayed a 5-fold higher V_max(app) at both pH 8.0 and 7.3 (Table 2). Because the RcPPC4 and AtPPC3 subunits occur in a 1:1 stoichiometric ratio within the Class-2 complex (Figs. 1C and and22C), their relative catalytic efficiencies were compared as V_max(app)/K_m_(PEP) (Table 2). The low K_m site (attributed to AtPPC3) was 3- and 3.5-fold more efficient at pH 7.3 and 8.0, respectively.

TABLE 2

PEP saturation kinetics of recombinant PEPCs

The standard spectrophotometric assay was used except that the PEP concentration was varied. Apparent V_max and K_m(PEP) values represent the mean of four separate determinations and are reproducible within ±15% (S.E.) of the mean, with the exception of the values for the Class-2 PEPC low K_m site, which have an error of approximately ±30% (S.E.).

Assay	Class-1 PEPC (AtPPC3)			RcPPC4			Class-2 PEPC (AtPPC3 + RcPPC4)
pH	V_max	K_m	V_max/K_m	V_max	K_m	V_max/K_m	V_max1^{^a}	K_m1	V_max1/K_m₁	V_max2^{^a}	K_m₂	V_max2/K_m₂
	units/mg	μm	units/mg μm⁻¹	units/mg	μm	units/mg μm⁻¹	units/mg	μm	units/mg μm⁻¹	units/mg	μm	units/mg μm⁻¹
7.3	15.4	77	0.20	26.7	840	0.032	4.0	38	0.11	21.4	580	0.037
8.0	15.1	65	0.23	28.0	870	0.032	4.5	34	0.13	20.7	560	0.037

^V_max1 and V_max2 values are listed as units/mg of Class-2 PEPC. As AtPPC3 and RcPPC4 occur in a 1:1 stoichiometric ratio in the Class-2 PEPC, the V_max(app) of individual AtPPC3 and RcPPC4 subunits within the Class-2 PEPC complex can be estimated by doubling V_max1 and V_max2, respectively.

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FIGURE 6.

Analysis of the biphasic PEP saturation kinetics of a chimeric Class-2 PEPC.A, PEP saturation curves were determined at pH 7.3 and fitted to either a single catalytic site Michaelis-Menten model (dashed line) or a biphasic model with two distinct catalytic sites (solid line) and plotted as Eadie-Hofstee linearizations. Inset, plot of the reaction rate versus PEP concentration. B, PEP saturation curves were determined at pH 7.0 in the presence of 0 (●), 0.2 (Δ), 0.5 (□), and 2 mm (○) malate and analyzed as above. Inset, relationship between V_max(app) values and malate concentration. V_max1 (●) and V_max2 (○) correspond to the V_max(app) values obtained for the low (AtPPC3) and high (RcPPC4) K_m_(PEP) sites, respectively, as discussed in the text. All data represent the mean ± S.E. of four separate determinations.

Metabolic Effectors

A variety of compounds were tested as possible effectors of the three recombinant PEPC isoforms at pH 8.0 and 7.0 with subsaturating PEP (0.2 mm). At pH 8.0 there was a negligible effect (±15% of the control rate) by glucose 6-P, fructose 6-P, glycerol 3-P, malate, Asp, or Glu (5 mm each) on the activity of AtPPC3, RcPPC4, or the chimeric Class-2 PEPC. However, as previously documented for a variety of plant Class-1 PEPCs including the developing COS Class-1 PEPC (RcPPC3) (1, 10, 13), AtPPC3 was significantly activated by hexose monophosphates and glycerol 3-P, and potently inhibited by malate and Asp when assayed at pH 7.0 (Table 3). Interestingly, RcPPC4 activity was unresponsive (±10% of the control rate) to 25 mm Glc-6-P, Fru-6-P, or glycerol 3-P, whereas its IC₅₀ values for Asp and malate were, respectively, 34- and 69-fold greater than those obtained for AtPPC3 under identical assay conditions (Table 3). The following metabolites had no detectable effect on RcPPC4 activity (tested at pH 7.0 and subsaturating PEP): 50 mm Gly; citrate, isocitrate, ribose 5-P, gluconate 6-P, succinate, fumarate, pyruvate, acetate, fructose 1,6-P₂, ATP, ADP, AMP, PP_i, P_i, Gln, Asn, Ser, Arg, Ala, and Cys (5 mm each); 1 mm shikimic acid; dihydroxyacetone-P and acetyl-CoA (0.5 mm each); and rutin and quercitin (0.1 mm each). Preincubation with 5 mm oxidized glutathione for up to 10 min at 30 °C also had no influence on RcPPC4 activity. Relative to AtPPC3, the activity of the chimeric Class-2 PEPC was significantly less affected by hexose mono-Ps, glycerol 3-P, malate, and Asp (Table 3). These results are reminiscent of analogous comparisons of green algal Class-1 and Class-2 PEPCs (15, 16), as well native Class-1 PEPC and partially degraded Class-2 PEPC (containing proteolytic truncated RcPPC4) from developing COS (10, 13).

TABLE 3

Influence of selected metabolites on the activity of recombinant PEPCs

The PEPC activity was determined at pH 7.0 with subsaturating PEP (0.2 mm) in the presence of each effector at 2 mm. Activities are expressed relative to the respective control determined in the absence of any additions and set at 100%. Shown in parentheses are IC₅₀(malate, Asp) values determined at pH 7.0 with 0.2 mm PEP. All values represent the mean of four separate determinations and are reproducible within ± 10% (S.E.) of the mean value.

Addition	Relative activity
Addition	Class-1 PEPC AtPPC3	RcPPC4	Class-2 PEPC AtPPC3/RcPPC4
Glc-6-P	191	93	121
Glc-1-P	171	95	114
Fru-6-P	188	99	119
Glycerol 3-P	185	98	120
Malate	8.5 (0.16)^{^a}	84 (11)^{^a}	20 (0.56)^{^a}
Aspartate	36 (0.97)^{^a}	98 (33)^{^a}	63 (2.8)^{^a}

^a IC₅₀ values represent inhibitor concentration (mm) yielding 50% inhibition of PEPC activity.

The influence of the inhibitor malate on biphasic PEP binding of the Class-2 PEPC was assessed at pH 7.0 (Fig. 6B). As the malate concentration increased the contribution of the low K_m_(PEP) site to the overall activity of Class-2 PEPC was drastically reduced. Catalytic activity from the low K_m site was virtually absent in the presence of 2 mm malate, such that two distinct activities could not be resolved. The best fit for this curve was to the single catalytic site, Michaelis-Menten model. Conversely, 2 mm malate exerted a negligible effect on the V_max(app) of the high K_m site (Fig. 6B).

Effect of pH and Metal Cofactor Requirements

Similar to other plant PEPCs (1), the activity of the purified recombinant AtPPC3, RcPPC4, and chimeric Class-2 PEPC: (i) displayed a broad pH profile with optimal activity occurring in the pH 8.0–8.5 range (supplemental Fig. S3), and (ii) was absolutely dependent upon the presence of a bivalent metal cation cofactor, specifically Mg^{or Mn}^{. Metal ion requirements of RcPPC4 were investigated in more detail. It exhibited a 4-fold higher specific activity with saturating (10 m}m) Mg^{relative to Mn}^{, and a}K_m(Mg⁾ value of 0.10 ± 0.01 mm.

PEP Saturation Kinetics

RcPPC4 and AtPPC3 both displayed hyperbolic PEP saturation kinetics at pH 8.0 and physiological pH (7.3) (Table 2). However, the apparent K_m_(PEP) value of RcPPC4 at both pH values was about an order of magnitude greater than that of AtPPC3 whose K_m_(PEP) of approximately 70 μm is typical for a C₃ plant PEPC (1). PEP binding to the chimeric Class-2 PEPC was analyzed by fitting PEP saturation data to a single catalytic site Michaelis-Menten model or to a two-catalytic site Michaelian model using non-linear regression. The fit to the two-catalytic site model yielded a higher probability (p < 0.001). The biphasic PEP saturation kinetics of the Class-2 PEPC was visualized by Eadie-Hofstee plots of the kinetic data (Fig. 6A). The theoretical plot of the two-site model (solid line) fit the experimental points with a correlation coefficient of 0.99, whereas the plot obtained assuming a single catalytic site (dashed line) has a far lower correlation coefficient of 0.87. These results indicate that the RcPPC4 and AtPPC3 subunits are both catalytically active within the Class-2 PEPC, as previously inferred for a green algal Class-2 PEPC (17). The apparent K_m_(PEP) values for the two sites differed by approximately 15-fold with the high K_m site (attributed to RcPPC4) displayed a 5-fold higher V_max(app) at both pH 8.0 and 7.3 (Table 2). Because the RcPPC4 and AtPPC3 subunits occur in a 1:1 stoichiometric ratio within the Class-2 complex (Figs. 1C and and22C), their relative catalytic efficiencies were compared as V_max(app)/K_m_(PEP) (Table 2). The low K_m site (attributed to AtPPC3) was 3- and 3.5-fold more efficient at pH 7.3 and 8.0, respectively.

TABLE 2

PEP saturation kinetics of recombinant PEPCs

The standard spectrophotometric assay was used except that the PEP concentration was varied. Apparent V_max and K_m(PEP) values represent the mean of four separate determinations and are reproducible within ±15% (S.E.) of the mean, with the exception of the values for the Class-2 PEPC low K_m site, which have an error of approximately ±30% (S.E.).

Assay	Class-1 PEPC (AtPPC3)			RcPPC4			Class-2 PEPC (AtPPC3 + RcPPC4)
pH	V_max	K_m	V_max/K_m	V_max	K_m	V_max/K_m	V_max1^{^a}	K_m1	V_max1/K_m₁	V_max2^{^a}	K_m₂	V_max2/K_m₂
	units/mg	μm	units/mg μm⁻¹	units/mg	μm	units/mg μm⁻¹	units/mg	μm	units/mg μm⁻¹	units/mg	μm	units/mg μm⁻¹
7.3	15.4	77	0.20	26.7	840	0.032	4.0	38	0.11	21.4	580	0.037
8.0	15.1	65	0.23	28.0	870	0.032	4.5	34	0.13	20.7	560	0.037

^V_max1 and V_max2 values are listed as units/mg of Class-2 PEPC. As AtPPC3 and RcPPC4 occur in a 1:1 stoichiometric ratio in the Class-2 PEPC, the V_max(app) of individual AtPPC3 and RcPPC4 subunits within the Class-2 PEPC complex can be estimated by doubling V_max1 and V_max2, respectively.

FIGURE 6.

Analysis of the biphasic PEP saturation kinetics of a chimeric Class-2 PEPC.A, PEP saturation curves were determined at pH 7.3 and fitted to either a single catalytic site Michaelis-Menten model (dashed line) or a biphasic model with two distinct catalytic sites (solid line) and plotted as Eadie-Hofstee linearizations. Inset, plot of the reaction rate versus PEP concentration. B, PEP saturation curves were determined at pH 7.0 in the presence of 0 (●), 0.2 (Δ), 0.5 (□), and 2 mm (○) malate and analyzed as above. Inset, relationship between V_max(app) values and malate concentration. V_max1 (●) and V_max2 (○) correspond to the V_max(app) values obtained for the low (AtPPC3) and high (RcPPC4) K_m_(PEP) sites, respectively, as discussed in the text. All data represent the mean ± S.E. of four separate determinations.

Effect of pH and Metal Cofactor Requirements

Similar to other plant PEPCs (1), the activity of the purified recombinant AtPPC3, RcPPC4, and chimeric Class-2 PEPC: (i) displayed a broad pH profile with optimal activity occurring in the pH 8.0–8.5 range (supplemental Fig. S3), and (ii) was absolutely dependent upon the presence of a bivalent metal cation cofactor, specifically Mg^{or Mn}^{. Metal ion requirements of RcPPC4 were investigated in more detail. It exhibited a 4-fold higher specific activity with saturating (10 m}m) Mg^{relative to Mn}^{, and a}K_m(Mg⁾ value of 0.10 ± 0.01 mm.

PEP Saturation Kinetics

RcPPC4 and AtPPC3 both displayed hyperbolic PEP saturation kinetics at pH 8.0 and physiological pH (7.3) (Table 2). However, the apparent K_m_(PEP) value of RcPPC4 at both pH values was about an order of magnitude greater than that of AtPPC3 whose K_m_(PEP) of approximately 70 μm is typical for a C₃ plant PEPC (1). PEP binding to the chimeric Class-2 PEPC was analyzed by fitting PEP saturation data to a single catalytic site Michaelis-Menten model or to a two-catalytic site Michaelian model using non-linear regression. The fit to the two-catalytic site model yielded a higher probability (p < 0.001). The biphasic PEP saturation kinetics of the Class-2 PEPC was visualized by Eadie-Hofstee plots of the kinetic data (Fig. 6A). The theoretical plot of the two-site model (solid line) fit the experimental points with a correlation coefficient of 0.99, whereas the plot obtained assuming a single catalytic site (dashed line) has a far lower correlation coefficient of 0.87. These results indicate that the RcPPC4 and AtPPC3 subunits are both catalytically active within the Class-2 PEPC, as previously inferred for a green algal Class-2 PEPC (17). The apparent K_m_(PEP) values for the two sites differed by approximately 15-fold with the high K_m site (attributed to RcPPC4) displayed a 5-fold higher V_max(app) at both pH 8.0 and 7.3 (Table 2). Because the RcPPC4 and AtPPC3 subunits occur in a 1:1 stoichiometric ratio within the Class-2 complex (Figs. 1C and and22C), their relative catalytic efficiencies were compared as V_max(app)/K_m_(PEP) (Table 2). The low K_m site (attributed to AtPPC3) was 3- and 3.5-fold more efficient at pH 7.3 and 8.0, respectively.

TABLE 2

PEP saturation kinetics of recombinant PEPCs

The standard spectrophotometric assay was used except that the PEP concentration was varied. Apparent V_max and K_m(PEP) values represent the mean of four separate determinations and are reproducible within ±15% (S.E.) of the mean, with the exception of the values for the Class-2 PEPC low K_m site, which have an error of approximately ±30% (S.E.).

Assay	Class-1 PEPC (AtPPC3)			RcPPC4			Class-2 PEPC (AtPPC3 + RcPPC4)
pH	V_max	K_m	V_max/K_m	V_max	K_m	V_max/K_m	V_max1^{^a}	K_m1	V_max1/K_m₁	V_max2^{^a}	K_m₂	V_max2/K_m₂
	units/mg	μm	units/mg μm⁻¹	units/mg	μm	units/mg μm⁻¹	units/mg	μm	units/mg μm⁻¹	units/mg	μm	units/mg μm⁻¹
7.3	15.4	77	0.20	26.7	840	0.032	4.0	38	0.11	21.4	580	0.037
8.0	15.1	65	0.23	28.0	870	0.032	4.5	34	0.13	20.7	560	0.037

^V_max1 and V_max2 values are listed as units/mg of Class-2 PEPC. As AtPPC3 and RcPPC4 occur in a 1:1 stoichiometric ratio in the Class-2 PEPC, the V_max(app) of individual AtPPC3 and RcPPC4 subunits within the Class-2 PEPC complex can be estimated by doubling V_max1 and V_max2, respectively.

FIGURE 6.

Analysis of the biphasic PEP saturation kinetics of a chimeric Class-2 PEPC.A, PEP saturation curves were determined at pH 7.3 and fitted to either a single catalytic site Michaelis-Menten model (dashed line) or a biphasic model with two distinct catalytic sites (solid line) and plotted as Eadie-Hofstee linearizations. Inset, plot of the reaction rate versus PEP concentration. B, PEP saturation curves were determined at pH 7.0 in the presence of 0 (●), 0.2 (Δ), 0.5 (□), and 2 mm (○) malate and analyzed as above. Inset, relationship between V_max(app) values and malate concentration. V_max1 (●) and V_max2 (○) correspond to the V_max(app) values obtained for the low (AtPPC3) and high (RcPPC4) K_m_(PEP) sites, respectively, as discussed in the text. All data represent the mean ± S.E. of four separate determinations.

Assay	Class-1 PEPC (AtPPC3)	RcPPC4	Class-2 PEPC (AtPPC3 + RcPPC4)
	units/mg	μm	units/mg μm⁻¹	units/mg	μm	units/mg μm⁻¹	units/mg	μm	units/mg μm⁻¹	units/mg	μm	units/mg μm⁻¹
7.3	15.4	77	0.20	26.7	840	0.032	4.0	38	0.11	21.4	580	0.037
8.0	15.1	65	0.23	28.0	870	0.032	4.5	34	0.13	20.7	560	0.037

Addition	Relative activity
Glc-6-P	191	93	121
Glc-1-P	171	95	114
Fru-6-P	188	99	119
Glycerol 3-P	185	98	120
Malate	8.5 (0.16)^{^a}	84 (11)^{^a}	20 (0.56)^{^a}
Aspartate	36 (0.97)^{^a}	98 (33)^{^a}	63 (2.8)^{^a}

Recombinant RcPPC4 Exhibits PEPC Activity

To determine whether RcPpc4 encodes a functional PEPC, the corresponding cDNA was subcloned into the E. coli expression vector pET28b and transformed into the expression strain BL21(DE3). The expressed recombinant RcPPC4 was fully purified from clarified extracts as an active PEPC that exhibited unusual physical and kinetic properties. The specific activity of purified RcPPC4 was 27 units/mg, which compares favorably with that of PEPCs isolated from other plant and algal sources, including the value of 22 units/mg reported for purified recombinant BTPC from the green alga C. reinhardtii (20). The high specific activity of RcPPC4 is consistent with the existence in deduced vascular plant BTPC sequences of all conserved subdomains that contribute residues believed to be essential for PEPC catalysis (supplemental Fig. S1) (11, 23). Acetyl-CoA, a potent allosteric activator of many bacterial PEPCs, but not of the vascular plant or green algal enzymes (1), exerted no effect on RcPPC4 activity. Together with the SDS-PAGE and parallel immunoblot analyses shown in Fig. 2 (panels A and B) this indicates that the purified RcPPC4 was free of any contaminating PEPC originating from the BL21(DE3) host cells. Compared with native and recombinant Class-1 PEPCs (10, 17), RcPPC4 displayed enhanced thermal stability (Fig. 5), an increased K_m_(PEP) (Table 2), and a noteworthy insensitivity to allosteric effectors. No allosteric activators of RcPPC4 were identified, whereas effective inhibition by malate and Asp only occurred at relatively high concentrations (Table 3). RcPPC4 retains all conserved residues known to bind the effectors malate and Asp, but its insensitivity toward hexose mono-Ps reflects the conspicuous absence in deduced BTPC sequences of two conserved PTPC-specific loops (348–369 and 914–950, maize C₄ PEPC numbering) that are proximal to the Glc-6-P binding site and potentially involved in accommodating the sugar moiety (supplemental Fig. S1) (23, 31, 32).

Recombinant RcPPC4 Forms Class-2 PEPCs When Combined with Class-1 PEPCs

Although COS and green algal BTPCs are catalytically active several independent lines of evidence strongly suggest that the native BTPCs only exist in vivo in physical association with corresponding PTPC subunits in a Class-2 PEPC enzyme complex (10, 11, 13 –18, 19). It is notable that throughout its purification the recombinant RcPPC4 tends to self-associate into large aggregates of limited solubility, which precluded the use of biophysical techniques for assessing its interaction with Class-1 PEPCs. However, upon mixing freshly prepared E. coli extracts containing heterologously expressed RcPPC4 with an excess of recombinant AtPPC3, RcPPC4 was quantitatively bound by AtPPC3 to form a 900-kDa Class-2 PEPC hetero-octameric complex composed of an equivalent ratio of RcPPC4:AtPPC3 subunits (Figs. 1, B and C; ;2,2, C, D, and E; and and44E). All problems pertaining to the insolubility and instability of RcPPC4 during its purification and storage were thereby eliminated, and the chimeric Class-2 PEPC complex never dissociated into its component subunits under non-denaturing conditions. Furthermore, the purified RcPPC4 could only be resolved as a discrete PEPC activity or anti-RcPPC4-IgG immunoreactive band during native PAGE after it was mixed with a Class-1 PEPC (Fig. 4). Native Class-1 PEPCs from developing COS and various vascular plant sources all formed high M_r Class-2 PEPCs when preincubated with the purified RcPPC4 (Fig. 4). The quantitative co-IP of native Class-2 PEPC from clarified COS extracts on an anti-RcPPC4-IgG immunoaffinity column (Fig. 3) corroborated our earlier study that exploited anti-RcPPC3-IgG to co-IP Class-1 and Class-2 PEPCs from developing COS extracts (14). Collective findings of our current and earlier studies support the hypothesis that PTPCs are compulsory and physiologically relevant binding partners for BTPCs that play an indispensable in vivo role in maintaining BTPC in its proper structural and functional state in Class-2 PEPCs.

RcPPC4 Functions as a Catalytic and Regulatory Subunit of the Class-2 PEPC Complex

The final specific activity of the chimeric Class-2 PEPC (25 units/mg; Table 1) was more than twice that obtained with the purified native COS Class-2 PEPC containing proteolytically cleaved RcPPC4 (10.3 units/mg) (10). This suggests that the RcPPC4 subunits were catalytically active in the recombinant Class-2 PEPC. Indeed, the recombinant Class-2 PEPC displayed biphasic PEP saturation kinetics (Fig. 6, Table 2) indicating the presence of distinct catalytic sites. This phenomenon was previously documented with the purified non-proteolyzed native Class-2 PEPC from the green alga S. minutum in which the high affinity site exhibited V_max(app) and K_m_(PEP) values (7.3 units/mg, 0.13 mm) that were, respectively, about 4- and 10-fold lower than those of the low affinity site (17). These relationships are very similar to those obtained in the current study (Table 2). The high and low K_m_(PEP) sites of S. minutum Class-2 PEPC were attributed to its PTPC and BTPC catalytic subunits, respectively (17). By contrast, three lines of evidence demonstrate that the high and low K_m_(PEP) sites of the chimeric Class-2 PEPC studied here, respectively, arise from its RcPPC4 (BTPC) and AtPPC3 (PTPC) catalytic subunits: (i) the relevant K_m_(PEP) values were in the same range as those obtained for the individually purified RcPPC4 and AtPPC3 (Table 2); (ii) 2 mm malate caused a pronounced decrease in the V_max(app) of the low, but not high K_m_(PEP) site (Fig. 6B), consistent with purified AtPPC3 exhibiting an IC_50(malate) value that was over 60-fold lower than that of the RcPPC4 (Table 3); and (iii) purified native COS Class-2 PEPC containing proteolytically cleaved (inactive) RcPPC4 exhibited classical Michaelis-Menten PEP saturation kinetics with a K_m_(PEP) of 60 μm at pH 8.0, equivalent to the values determined for the corresponding native RcPPC3 (10), as well as the purified recombinant AtPPC3 examined in the current study (Table 2).

Our kinetic analyses also revealed that the physical interaction between the AtPPC3 and RcPPC4 polypeptides resulted in kinetic activation of both PEPC subunits within the chimeric Class-2 PEPC. It is notable that the K_m_(PEP) value for AtPPC3 was reduced by about 2-fold upon entering into the Class-2 PEPC complex, whereas that of RcPPC4 decreased by about one-third (Table 2). The corresponding V_max(app) values also appeared to shift. As AtPPC3 and RcPPC4 occur in a 1:1 ratio within the Class-2 PEPC, one can estimate that the V_max(app) of its AtPPC3 subunits was actually reduced by about 40% in the complex (from approximately 15 to 9 units/mg of AtPPC3), whereas that of the RcPPC4 subunits was increased by 56% (from approximately 27 to 42 units/mg of RcPPC4) (Table 2). Furthermore, the Class-2 PEPC demonstrated significantly lower sensitivity to allosteric activators (hexose mono-Ps and glycerol 3-P) and inhibitors (malate and Asp) relative to AtPPC3 (Table 3). This agrees with previous studies that have documented the marked insensitivity of native green algal and COS Class-2 PEPC complexes to allosteric effectors, relative to the corresponding Class-1 PEPC (10, 13, 15, 16). Similar to native Class-2 PEPC complexes from green algae and developing COS (10, 15), the chimeric Class-2 PEPC displayed enhanced thermal stability relative to the corresponding Class-1 PEPC (Fig. 5). Results of the present study also corroborate previous work indicating that the tight interaction between RcPPC4 and RcPPC3 subunits in the castor Class-2 PEPC is not influenced by their respective phosphorylation status (13, 14). Nevertheless, in vitro dephosphorylation by the catalytic subunit of bovine protein phosphatase type 2A exerted a reciprocal influence on the activity of purified native Class-1 PEPC (inhibited) and partially truncated Class-2 PEPC (activated) from developing COS (13). Determining the influence that in vivo multisite BTPC phosphorylation (14) has on the structural and functional properties COS Class-2 PEPC is the subject of ongoing research.

Concluding Remarks

We have provided new insights into the biochemistry of the castor plant BTPC, RcPPC4, and its function as one of two distinct PEPC catalytic subunit types in the unusual Class-2 PEPC enzyme complex of vascular plants. PEPC has been hypothesized to operate at a key branch point in the control of C-partitioning to storage oil and protein in developing seeds. However, it remains to be determined why two distinct oligomeric classes of PEPC are specifically and highly expressed in developing COS. It is apparent that BTPCs readily and tightly bind to Class-1 PEPCs to create unique Class-2 PEPC hetero-oligomers that exhibit altered physical, kinetic, and regulatory properties. The kinetic and regulatory features of the Class-2 PEPC complex are consistent with its putative function as a “metabolic overflow” mechanism that could maintain a significant flux from PEP to malate under physiological conditions that would largely inhibit a Class-1 PEPC. However, we cannot exclude additional functions for Class-2 PEPCs, such as mediating a specific subcellular location and/or further protein-protein interactions (14). It will also be of interest to establish a structural model for plant and algal Class-2 PEPC subunit architecture, and the location of conserved binding domains between their PTPC and BTPC subunits. Patterns of BTPC and Class-2 PEPC expression in various plant species and tissues also need to be re-evaluated together with functional genomic approaches to assess the impact of altered BTPC expression on C-partitioning and carbon-nitrogen interactions in developing seeds.

Recombinant RcPPC4 Exhibits PEPC Activity

To determine whether RcPpc4 encodes a functional PEPC, the corresponding cDNA was subcloned into the E. coli expression vector pET28b and transformed into the expression strain BL21(DE3). The expressed recombinant RcPPC4 was fully purified from clarified extracts as an active PEPC that exhibited unusual physical and kinetic properties. The specific activity of purified RcPPC4 was 27 units/mg, which compares favorably with that of PEPCs isolated from other plant and algal sources, including the value of 22 units/mg reported for purified recombinant BTPC from the green alga C. reinhardtii (20). The high specific activity of RcPPC4 is consistent with the existence in deduced vascular plant BTPC sequences of all conserved subdomains that contribute residues believed to be essential for PEPC catalysis (supplemental Fig. S1) (11, 23). Acetyl-CoA, a potent allosteric activator of many bacterial PEPCs, but not of the vascular plant or green algal enzymes (1), exerted no effect on RcPPC4 activity. Together with the SDS-PAGE and parallel immunoblot analyses shown in Fig. 2 (panels A and B) this indicates that the purified RcPPC4 was free of any contaminating PEPC originating from the BL21(DE3) host cells. Compared with native and recombinant Class-1 PEPCs (10, 17), RcPPC4 displayed enhanced thermal stability (Fig. 5), an increased K_m_(PEP) (Table 2), and a noteworthy insensitivity to allosteric effectors. No allosteric activators of RcPPC4 were identified, whereas effective inhibition by malate and Asp only occurred at relatively high concentrations (Table 3). RcPPC4 retains all conserved residues known to bind the effectors malate and Asp, but its insensitivity toward hexose mono-Ps reflects the conspicuous absence in deduced BTPC sequences of two conserved PTPC-specific loops (348–369 and 914–950, maize C₄ PEPC numbering) that are proximal to the Glc-6-P binding site and potentially involved in accommodating the sugar moiety (supplemental Fig. S1) (23, 31, 32).

Recombinant RcPPC4 Forms Class-2 PEPCs When Combined with Class-1 PEPCs

Although COS and green algal BTPCs are catalytically active several independent lines of evidence strongly suggest that the native BTPCs only exist in vivo in physical association with corresponding PTPC subunits in a Class-2 PEPC enzyme complex (10, 11, 13 –18, 19). It is notable that throughout its purification the recombinant RcPPC4 tends to self-associate into large aggregates of limited solubility, which precluded the use of biophysical techniques for assessing its interaction with Class-1 PEPCs. However, upon mixing freshly prepared E. coli extracts containing heterologously expressed RcPPC4 with an excess of recombinant AtPPC3, RcPPC4 was quantitatively bound by AtPPC3 to form a 900-kDa Class-2 PEPC hetero-octameric complex composed of an equivalent ratio of RcPPC4:AtPPC3 subunits (Figs. 1, B and C; ;2,2, C, D, and E; and and44E). All problems pertaining to the insolubility and instability of RcPPC4 during its purification and storage were thereby eliminated, and the chimeric Class-2 PEPC complex never dissociated into its component subunits under non-denaturing conditions. Furthermore, the purified RcPPC4 could only be resolved as a discrete PEPC activity or anti-RcPPC4-IgG immunoreactive band during native PAGE after it was mixed with a Class-1 PEPC (Fig. 4). Native Class-1 PEPCs from developing COS and various vascular plant sources all formed high M_r Class-2 PEPCs when preincubated with the purified RcPPC4 (Fig. 4). The quantitative co-IP of native Class-2 PEPC from clarified COS extracts on an anti-RcPPC4-IgG immunoaffinity column (Fig. 3) corroborated our earlier study that exploited anti-RcPPC3-IgG to co-IP Class-1 and Class-2 PEPCs from developing COS extracts (14). Collective findings of our current and earlier studies support the hypothesis that PTPCs are compulsory and physiologically relevant binding partners for BTPCs that play an indispensable in vivo role in maintaining BTPC in its proper structural and functional state in Class-2 PEPCs.

RcPPC4 Functions as a Catalytic and Regulatory Subunit of the Class-2 PEPC Complex

The final specific activity of the chimeric Class-2 PEPC (25 units/mg; Table 1) was more than twice that obtained with the purified native COS Class-2 PEPC containing proteolytically cleaved RcPPC4 (10.3 units/mg) (10). This suggests that the RcPPC4 subunits were catalytically active in the recombinant Class-2 PEPC. Indeed, the recombinant Class-2 PEPC displayed biphasic PEP saturation kinetics (Fig. 6, Table 2) indicating the presence of distinct catalytic sites. This phenomenon was previously documented with the purified non-proteolyzed native Class-2 PEPC from the green alga S. minutum in which the high affinity site exhibited V_max(app) and K_m_(PEP) values (7.3 units/mg, 0.13 mm) that were, respectively, about 4- and 10-fold lower than those of the low affinity site (17). These relationships are very similar to those obtained in the current study (Table 2). The high and low K_m_(PEP) sites of S. minutum Class-2 PEPC were attributed to its PTPC and BTPC catalytic subunits, respectively (17). By contrast, three lines of evidence demonstrate that the high and low K_m_(PEP) sites of the chimeric Class-2 PEPC studied here, respectively, arise from its RcPPC4 (BTPC) and AtPPC3 (PTPC) catalytic subunits: (i) the relevant K_m_(PEP) values were in the same range as those obtained for the individually purified RcPPC4 and AtPPC3 (Table 2); (ii) 2 mm malate caused a pronounced decrease in the V_max(app) of the low, but not high K_m_(PEP) site (Fig. 6B), consistent with purified AtPPC3 exhibiting an IC_50(malate) value that was over 60-fold lower than that of the RcPPC4 (Table 3); and (iii) purified native COS Class-2 PEPC containing proteolytically cleaved (inactive) RcPPC4 exhibited classical Michaelis-Menten PEP saturation kinetics with a K_m_(PEP) of 60 μm at pH 8.0, equivalent to the values determined for the corresponding native RcPPC3 (10), as well as the purified recombinant AtPPC3 examined in the current study (Table 2).

Our kinetic analyses also revealed that the physical interaction between the AtPPC3 and RcPPC4 polypeptides resulted in kinetic activation of both PEPC subunits within the chimeric Class-2 PEPC. It is notable that the K_m_(PEP) value for AtPPC3 was reduced by about 2-fold upon entering into the Class-2 PEPC complex, whereas that of RcPPC4 decreased by about one-third (Table 2). The corresponding V_max(app) values also appeared to shift. As AtPPC3 and RcPPC4 occur in a 1:1 ratio within the Class-2 PEPC, one can estimate that the V_max(app) of its AtPPC3 subunits was actually reduced by about 40% in the complex (from approximately 15 to 9 units/mg of AtPPC3), whereas that of the RcPPC4 subunits was increased by 56% (from approximately 27 to 42 units/mg of RcPPC4) (Table 2). Furthermore, the Class-2 PEPC demonstrated significantly lower sensitivity to allosteric activators (hexose mono-Ps and glycerol 3-P) and inhibitors (malate and Asp) relative to AtPPC3 (Table 3). This agrees with previous studies that have documented the marked insensitivity of native green algal and COS Class-2 PEPC complexes to allosteric effectors, relative to the corresponding Class-1 PEPC (10, 13, 15, 16). Similar to native Class-2 PEPC complexes from green algae and developing COS (10, 15), the chimeric Class-2 PEPC displayed enhanced thermal stability relative to the corresponding Class-1 PEPC (Fig. 5). Results of the present study also corroborate previous work indicating that the tight interaction between RcPPC4 and RcPPC3 subunits in the castor Class-2 PEPC is not influenced by their respective phosphorylation status (13, 14). Nevertheless, in vitro dephosphorylation by the catalytic subunit of bovine protein phosphatase type 2A exerted a reciprocal influence on the activity of purified native Class-1 PEPC (inhibited) and partially truncated Class-2 PEPC (activated) from developing COS (13). Determining the influence that in vivo multisite BTPC phosphorylation (14) has on the structural and functional properties COS Class-2 PEPC is the subject of ongoing research.

Concluding Remarks

We have provided new insights into the biochemistry of the castor plant BTPC, RcPPC4, and its function as one of two distinct PEPC catalytic subunit types in the unusual Class-2 PEPC enzyme complex of vascular plants. PEPC has been hypothesized to operate at a key branch point in the control of C-partitioning to storage oil and protein in developing seeds. However, it remains to be determined why two distinct oligomeric classes of PEPC are specifically and highly expressed in developing COS. It is apparent that BTPCs readily and tightly bind to Class-1 PEPCs to create unique Class-2 PEPC hetero-oligomers that exhibit altered physical, kinetic, and regulatory properties. The kinetic and regulatory features of the Class-2 PEPC complex are consistent with its putative function as a “metabolic overflow” mechanism that could maintain a significant flux from PEP to malate under physiological conditions that would largely inhibit a Class-1 PEPC. However, we cannot exclude additional functions for Class-2 PEPCs, such as mediating a specific subcellular location and/or further protein-protein interactions (14). It will also be of interest to establish a structural model for plant and algal Class-2 PEPC subunit architecture, and the location of conserved binding domains between their PTPC and BTPC subunits. Patterns of BTPC and Class-2 PEPC expression in various plant species and tissues also need to be re-evaluated together with functional genomic approaches to assess the impact of altered BTPC expression on C-partitioning and carbon-nitrogen interactions in developing seeds.

Recombinant RcPPC4 Exhibits PEPC Activity

To determine whether RcPpc4 encodes a functional PEPC, the corresponding cDNA was subcloned into the E. coli expression vector pET28b and transformed into the expression strain BL21(DE3). The expressed recombinant RcPPC4 was fully purified from clarified extracts as an active PEPC that exhibited unusual physical and kinetic properties. The specific activity of purified RcPPC4 was 27 units/mg, which compares favorably with that of PEPCs isolated from other plant and algal sources, including the value of 22 units/mg reported for purified recombinant BTPC from the green alga C. reinhardtii (20). The high specific activity of RcPPC4 is consistent with the existence in deduced vascular plant BTPC sequences of all conserved subdomains that contribute residues believed to be essential for PEPC catalysis (supplemental Fig. S1) (11, 23). Acetyl-CoA, a potent allosteric activator of many bacterial PEPCs, but not of the vascular plant or green algal enzymes (1), exerted no effect on RcPPC4 activity. Together with the SDS-PAGE and parallel immunoblot analyses shown in Fig. 2 (panels A and B) this indicates that the purified RcPPC4 was free of any contaminating PEPC originating from the BL21(DE3) host cells. Compared with native and recombinant Class-1 PEPCs (10, 17), RcPPC4 displayed enhanced thermal stability (Fig. 5), an increased K_m_(PEP) (Table 2), and a noteworthy insensitivity to allosteric effectors. No allosteric activators of RcPPC4 were identified, whereas effective inhibition by malate and Asp only occurred at relatively high concentrations (Table 3). RcPPC4 retains all conserved residues known to bind the effectors malate and Asp, but its insensitivity toward hexose mono-Ps reflects the conspicuous absence in deduced BTPC sequences of two conserved PTPC-specific loops (348–369 and 914–950, maize C₄ PEPC numbering) that are proximal to the Glc-6-P binding site and potentially involved in accommodating the sugar moiety (supplemental Fig. S1) (23, 31, 32).

Recombinant RcPPC4 Forms Class-2 PEPCs When Combined with Class-1 PEPCs

Although COS and green algal BTPCs are catalytically active several independent lines of evidence strongly suggest that the native BTPCs only exist in vivo in physical association with corresponding PTPC subunits in a Class-2 PEPC enzyme complex (10, 11, 13 –18, 19). It is notable that throughout its purification the recombinant RcPPC4 tends to self-associate into large aggregates of limited solubility, which precluded the use of biophysical techniques for assessing its interaction with Class-1 PEPCs. However, upon mixing freshly prepared E. coli extracts containing heterologously expressed RcPPC4 with an excess of recombinant AtPPC3, RcPPC4 was quantitatively bound by AtPPC3 to form a 900-kDa Class-2 PEPC hetero-octameric complex composed of an equivalent ratio of RcPPC4:AtPPC3 subunits (Figs. 1, B and C; ;2,2, C, D, and E; and and44E). All problems pertaining to the insolubility and instability of RcPPC4 during its purification and storage were thereby eliminated, and the chimeric Class-2 PEPC complex never dissociated into its component subunits under non-denaturing conditions. Furthermore, the purified RcPPC4 could only be resolved as a discrete PEPC activity or anti-RcPPC4-IgG immunoreactive band during native PAGE after it was mixed with a Class-1 PEPC (Fig. 4). Native Class-1 PEPCs from developing COS and various vascular plant sources all formed high M_r Class-2 PEPCs when preincubated with the purified RcPPC4 (Fig. 4). The quantitative co-IP of native Class-2 PEPC from clarified COS extracts on an anti-RcPPC4-IgG immunoaffinity column (Fig. 3) corroborated our earlier study that exploited anti-RcPPC3-IgG to co-IP Class-1 and Class-2 PEPCs from developing COS extracts (14). Collective findings of our current and earlier studies support the hypothesis that PTPCs are compulsory and physiologically relevant binding partners for BTPCs that play an indispensable in vivo role in maintaining BTPC in its proper structural and functional state in Class-2 PEPCs.

RcPPC4 Functions as a Catalytic and Regulatory Subunit of the Class-2 PEPC Complex

The final specific activity of the chimeric Class-2 PEPC (25 units/mg; Table 1) was more than twice that obtained with the purified native COS Class-2 PEPC containing proteolytically cleaved RcPPC4 (10.3 units/mg) (10). This suggests that the RcPPC4 subunits were catalytically active in the recombinant Class-2 PEPC. Indeed, the recombinant Class-2 PEPC displayed biphasic PEP saturation kinetics (Fig. 6, Table 2) indicating the presence of distinct catalytic sites. This phenomenon was previously documented with the purified non-proteolyzed native Class-2 PEPC from the green alga S. minutum in which the high affinity site exhibited V_max(app) and K_m_(PEP) values (7.3 units/mg, 0.13 mm) that were, respectively, about 4- and 10-fold lower than those of the low affinity site (17). These relationships are very similar to those obtained in the current study (Table 2). The high and low K_m_(PEP) sites of S. minutum Class-2 PEPC were attributed to its PTPC and BTPC catalytic subunits, respectively (17). By contrast, three lines of evidence demonstrate that the high and low K_m_(PEP) sites of the chimeric Class-2 PEPC studied here, respectively, arise from its RcPPC4 (BTPC) and AtPPC3 (PTPC) catalytic subunits: (i) the relevant K_m_(PEP) values were in the same range as those obtained for the individually purified RcPPC4 and AtPPC3 (Table 2); (ii) 2 mm malate caused a pronounced decrease in the V_max(app) of the low, but not high K_m_(PEP) site (Fig. 6B), consistent with purified AtPPC3 exhibiting an IC_50(malate) value that was over 60-fold lower than that of the RcPPC4 (Table 3); and (iii) purified native COS Class-2 PEPC containing proteolytically cleaved (inactive) RcPPC4 exhibited classical Michaelis-Menten PEP saturation kinetics with a K_m_(PEP) of 60 μm at pH 8.0, equivalent to the values determined for the corresponding native RcPPC3 (10), as well as the purified recombinant AtPPC3 examined in the current study (Table 2).

Our kinetic analyses also revealed that the physical interaction between the AtPPC3 and RcPPC4 polypeptides resulted in kinetic activation of both PEPC subunits within the chimeric Class-2 PEPC. It is notable that the K_m_(PEP) value for AtPPC3 was reduced by about 2-fold upon entering into the Class-2 PEPC complex, whereas that of RcPPC4 decreased by about one-third (Table 2). The corresponding V_max(app) values also appeared to shift. As AtPPC3 and RcPPC4 occur in a 1:1 ratio within the Class-2 PEPC, one can estimate that the V_max(app) of its AtPPC3 subunits was actually reduced by about 40% in the complex (from approximately 15 to 9 units/mg of AtPPC3), whereas that of the RcPPC4 subunits was increased by 56% (from approximately 27 to 42 units/mg of RcPPC4) (Table 2). Furthermore, the Class-2 PEPC demonstrated significantly lower sensitivity to allosteric activators (hexose mono-Ps and glycerol 3-P) and inhibitors (malate and Asp) relative to AtPPC3 (Table 3). This agrees with previous studies that have documented the marked insensitivity of native green algal and COS Class-2 PEPC complexes to allosteric effectors, relative to the corresponding Class-1 PEPC (10, 13, 15, 16). Similar to native Class-2 PEPC complexes from green algae and developing COS (10, 15), the chimeric Class-2 PEPC displayed enhanced thermal stability relative to the corresponding Class-1 PEPC (Fig. 5). Results of the present study also corroborate previous work indicating that the tight interaction between RcPPC4 and RcPPC3 subunits in the castor Class-2 PEPC is not influenced by their respective phosphorylation status (13, 14). Nevertheless, in vitro dephosphorylation by the catalytic subunit of bovine protein phosphatase type 2A exerted a reciprocal influence on the activity of purified native Class-1 PEPC (inhibited) and partially truncated Class-2 PEPC (activated) from developing COS (13). Determining the influence that in vivo multisite BTPC phosphorylation (14) has on the structural and functional properties COS Class-2 PEPC is the subject of ongoing research.

Concluding Remarks

We have provided new insights into the biochemistry of the castor plant BTPC, RcPPC4, and its function as one of two distinct PEPC catalytic subunit types in the unusual Class-2 PEPC enzyme complex of vascular plants. PEPC has been hypothesized to operate at a key branch point in the control of C-partitioning to storage oil and protein in developing seeds. However, it remains to be determined why two distinct oligomeric classes of PEPC are specifically and highly expressed in developing COS. It is apparent that BTPCs readily and tightly bind to Class-1 PEPCs to create unique Class-2 PEPC hetero-oligomers that exhibit altered physical, kinetic, and regulatory properties. The kinetic and regulatory features of the Class-2 PEPC complex are consistent with its putative function as a “metabolic overflow” mechanism that could maintain a significant flux from PEP to malate under physiological conditions that would largely inhibit a Class-1 PEPC. However, we cannot exclude additional functions for Class-2 PEPCs, such as mediating a specific subcellular location and/or further protein-protein interactions (14). It will also be of interest to establish a structural model for plant and algal Class-2 PEPC subunit architecture, and the location of conserved binding domains between their PTPC and BTPC subunits. Patterns of BTPC and Class-2 PEPC expression in various plant species and tissues also need to be re-evaluated together with functional genomic approaches to assess the impact of altered BTPC expression on C-partitioning and carbon-nitrogen interactions in developing seeds.

Supplemental Data:

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EXPERIMENTAL PROCEDURES

Cloning, Transformation, and Heterologous Expression of Recombinant PEPCs

Purification of Recombinant RcPPC4, AtPPC3, and Class-2 PEPC

Electrophoresis and Immunoblotting

Co-immunopurification

Enzyme and Protein Assays and Kinetic Studies

Statistics

Cloning, Transformation, and Heterologous Expression of Recombinant PEPCs

Purification of Recombinant RcPPC4, AtPPC3, and Class-2 PEPC

Electrophoresis and Immunoblotting

Co-immunopurification

Enzyme and Protein Assays and Kinetic Studies

Statistics

Cloning, Transformation, and Heterologous Expression of Recombinant PEPCs

Purification of Recombinant RcPPC4, AtPPC3, and Class-2 PEPC

Electrophoresis and Immunoblotting

Co-immunopurification

Enzyme and Protein Assays and Kinetic Studies

Statistics

Cloning, Transformation, and Heterologous Expression of Recombinant PEPCs

Purification of Recombinant RcPPC4, AtPPC3, and Class-2 PEPC

Electrophoresis and Immunoblotting

Co-immunopurification

Enzyme and Protein Assays and Kinetic Studies

Statistics

RESULTS

Heterologous Expression, Purification, and Immunological Characterization of the Bacterial-type PEP Carboxylase RcPPC4 from Developing Castor Oil Seeds

Recombinant RcPPC4 Forms Class-2 PEPC When Combined with Class-1 PEPCs

Purification of a Non-proteolyzed Chimeric Class-2 PEPC from Recombinant RcPPC4 and AtPPC3

TABLE 1

Kinetic Properties

Effect of pH and Metal Cofactor Requirements

PEP Saturation Kinetics

TABLE 2

Metabolic Effectors

TABLE 3

Heterologous Expression, Purification, and Immunological Characterization of the Bacterial-type PEP Carboxylase RcPPC4 from Developing Castor Oil Seeds

Recombinant RcPPC4 Forms Class-2 PEPC When Combined with Class-1 PEPCs

Purification of a Non-proteolyzed Chimeric Class-2 PEPC from Recombinant RcPPC4 and AtPPC3

TABLE 1

Kinetic Properties

Effect of pH and Metal Cofactor Requirements

PEP Saturation Kinetics

TABLE 2

Effect of pH and Metal Cofactor Requirements

PEP Saturation Kinetics

TABLE 2

Effect of pH and Metal Cofactor Requirements

PEP Saturation Kinetics

TABLE 2

Metabolic Effectors

TABLE 3

DISCUSSION

Recombinant RcPPC4 Exhibits PEPC Activity

Recombinant RcPPC4 Forms Class-2 PEPCs When Combined with Class-1 PEPCs

RcPPC4 Functions as a Catalytic and Regulatory Subunit of the Class-2 PEPC Complex

Concluding Remarks

Recombinant RcPPC4 Exhibits PEPC Activity

Recombinant RcPPC4 Forms Class-2 PEPCs When Combined with Class-1 PEPCs

RcPPC4 Functions as a Catalytic and Regulatory Subunit of the Class-2 PEPC Complex

Concluding Remarks

Recombinant RcPPC4 Exhibits PEPC Activity

Recombinant RcPPC4 Forms Class-2 PEPCs When Combined with Class-1 PEPCs

RcPPC4 Functions as a Catalytic and Regulatory Subunit of the Class-2 PEPC Complex

Concluding Remarks

Recombinant RcPPC4 Exhibits PEPC Activity

Recombinant RcPPC4 Forms Class-2 PEPCs When Combined with Class-1 PEPCs

RcPPC4 Functions as a Catalytic and Regulatory Subunit of the Class-2 PEPC Complex

Concluding Remarks

Supplementary Material

Abstract

REFERENCES

References