Titanium dioxide nanoparticles exhibit good anticancer and antibacterial activities. They are known to be environmentally friendly and stable, less toxic and excellent biocompatibility nature. In this paper we report the biological properties of pure TiO₂ nanoparticles modified with Withania somnifera (Ashwagandha), Eclipta prostrata (Karisalankanni) and Glycyrrhiza glabra (Athimathuram) for biological applications. X-ray diffraction results revealed the anatase nature of the samples. From the TEM analyses, it is observed that there is an increase in the particle size of the bio modified samples. UV results show the red shift for the bio modified samples when compared with the pure samples. The samples are then subjected to MTT assay to determine the cell viability. KB oral cancer cells are used for the determination of anticancer nature of the pure and bio modified nanoparticles. It is observed that Withania somnifera - Eclipta prostrate modified TiO₂ nanoparticles exhibit excellent anticancer activities among other bio modified and pure samples. The samples are then examined for their antibacterial activities against three Gram-negative bacterial strains namely, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and two Gram-positive bacterial strains namely, Staphylococcus aureus and Streptococcus mutans. Among the modified and pure samples, Withania somnifera - Eclipta prostrata showed good antibacterial nature against Gram-positive and Gram-negative bacteria.