Use of Protein in Extraction and Stabilization of Nitrate Reductase <sup><a href="#fn1" rid="fn1" class=" fn">1</a></sup>
Abstract
The in vitro instability of nitrate reductase (EC 1.6.6.1) activity from leaves of several species of higher plants was investigated. Decay of activity was exponential with time, suggesting that an enzyme-catalyzed reaction was involved. The rate of decay of nitrate reductase activity increased as leaf age increased in all species studied. Activity was relatively stable in certain genotypes of Zea mays L., but extremely unstable in others. In all genotypes of Avena sativa L. and Nicotiana tabacum L. studied, nitrate reductase was unstable. Addition of 3% (w/v) bovine serum albumin or casein to extraction media prevented or retarded the decay of nitrate reductase activity for several hours. In addition, the presence of bovine serum albumin or casein in the enzyme homogenate markedly increased nitrate reductase activity (up to 15-fold), especially in older leaf tissue.
Full text
Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (512K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Beevers L, Schrader LE, Flesher D, Hageman RH. The Role of Light and Nitrate in the Induction of Nitrate Reductase in Radish Cotyledons and Maize Seedlings. Plant Physiol. 1965 Jul;40(4):691–698.[PMC free article] [PubMed] [Google Scholar]
- Dalling MJ, Tolbert NE, Hageman RH. Intracellular location of nitrate reductase and nitrite reductase. I. Spinach and tobacco leaves. Biochim Biophys Acta. 1972 Dec 14;283(3):505–512. [PubMed] [Google Scholar]
- Warner RL, Hageman RH, Dudley JW, Lambert RJ. Inheritance of nitrate reductase activity in Zea mays L. Proc Natl Acad Sci U S A. 1969 Mar;62(3):785–792.[PMC free article] [PubMed] [Google Scholar]