In this work, isolation of the cellulose fibres was carried out via the Feedstock Impregnation Rapid and Sequential Steam Treatment process (FIRSST). The latter allows the separation of extractives, hemicellulosic sugars and lignin isolating the cellulose fibres. Quantitative data on the constitutive macromolecules of biomass was obtained using ASTM or TAPPI standard methods. Carbohydrates found in the hemicelluloses were also quantified using HPLC. Kraft pulp from whole biomass has also been produced at a bench scale (few kg per batch) using known and established pulping conditions. The pulps from both pulping techniques were tested following ATTPC standard methods. Pulp yields were of 34% for the classical Kraft processes (using whole biomass) while the FIRSST process showed yields around 30%. The average fibre lengths were similar for FIRSST pulp (0.39 mm) and Kraft pulp (0.41 mm) and the mechanical properties of the FIRSST pulp were as good as those of the Kraft pulp.