Mannan oligosaccharides trigger multiple defence responses in rice and tobacco as a novel danger-associated molecular pattern.
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Journal: 2019/May - Molecular Plant Pathology
ISSN: 1364-3703
Abstract:
Oligosaccharide, a typical danger-associated molecular pattern (DAMP), has been studied and applied as plant defence elicitor for several years. Here, we report a novel oligosaccharide, mannan oligosaccharide (MOS) with a degree of polymerization of 2-6, which was hydrolysed from locust bean gum by a newly reported enzyme, BpMan5. The MOS treatment can significantly enhance the generation of signalling molecules such as intracellular Ca2+ and reactive oxygen species. Subsequent defence events like stomata closure and cell death were also caused by MOS, eventually leading to the prevention of pathogen invasion or expansion. Transcriptional expression assay indicated that MOS activated mitogen-activated protein kinase cascades in tobacco and rice via different cascading pathways. The expression levels of the defence-related genes PR-1a and LOX were both up-regulated after MOS treatment, suggesting that MOS may simultaneously activate salicylic acid and jasmonic acid-dependent signalling pathways. Furthermore, liquid chromatography-mass spectrometry analysis showed that MOS led to the accumulation of four phytoalexins (momilactone A, phytocassane A, phytocassane D, and phytocassane E) in rice seedling leaves within 12-24 h. Finally, MOS conferred resistance in rice and tobacco against Xanthomonas oryzae and Phytophthora nicotianae, respectively. Taken together, our results indicated that MOS, a novel DAMP, could trigger multiple defence responses to prime plant resistance and has a great potential as plant defence elicitor for the management of plant disease.
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Mol Plant Pathol 20(8): 1067-1079
PMID: 31094073

Mannan oligosaccharides trigger multiple defence responses in rice and tobacco as a novel danger‐associated molecular pattern

Supporting information

Fig. S1 Expression and purification of BpMan5.

Fig. S2 Original image of intracellular Ca in guard cells of N. benthamiana.

Fig. S3 Original image of NO (A) and ROS (B) generation in guard cells of N. benthamiana.

Fig. S4 Original image of the stoma aperture in tobacco.

Table S1 Quantification of various kinds of oligosaccharides in a hydrolysis mixture.

Table S2 Primers designed for real time PCR experiment.

College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, 210095, PR China,
Institute of Plant Protection and Agro‐Products Safety, Anhui Academy of Agricultural Sciences, Hefei, 230031, China,
The National Key Engineering Lab of Crop Stress Resistance Breeding, College of Life Sciences, Anhui Agricultural University, Hefei, 230036, China,
Yu Chen, Email: moc.anis@19866uynehc.
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Corresponding author.
Correspondence: Emails: nc.ude.uajn@wxoag; moc.anis@19866uynehc,
Copyright © 2019 The Authors. Molecular Plant Pathology Published By British Society for Plant Pathology and John Wiley & Sons Ltd
This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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Notes

Co‐first authors: Haoyu Zang and Shanshan Xie; co‐corresponding authors: Xuewen Gao and Yu Chen.

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Yu Chen, Email: moc.anis@19866uynehc.

Xuewen Gao, Email: nc.ude.uajn@wxoag.

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