Biochem J 226(3): 679-684
Effect of sucrose starvation on sycamore (Acer pseudoplatanus) cell carbohydrate and Pi status.
Abstract
The mobilization of stored carbohydrates during sucrose starvation was studied with sycamore (Acer pseudoplatanus) cells. When sucrose was omitted from the nutrient medium, the intracellular sucrose pool decreased rapidly during the first hours of the experiment, whereas the starch content remained practically unchanged. After 10h of sucrose starvation, starch hydrolysis replaced sucrose breakdown. From this moment, the phosphate-ester pool and respiration rate decreased with time. Conversely, the intracellular Pi concentration increased. 31P n.m.r. of intact sycamore cells indicated that, under these conditions, most of the Pi accumulated in the vacuole. These results strongly suggest that starch breakdown, in contrast with sucrose hydrolysis, is not rapid enough to maintain a high cellular metabolism.
Full text
Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (916K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bligny R. Growth of Suspension-cultured Acer pseudoplatanus L. Cells in Automatic Culture Units of Large Volume. Plant Physiol. 1977 Mar;59(3):502–505.[PMC free article] [PubMed] [Google Scholar]
- Foyer C, Walker D, Spencer C, Mann B. Observations on the phosphate status and intracellular pH of intact cells, protoplasts and chloroplasts from photosynthetic tissue using phosphorus-31 nuclear magnetic resonance. Biochem J. 1982 Feb 15;202(2):429–434.[PMC free article] [PubMed] [Google Scholar]
- Heldt HW, Chon CJ, Maronde D. Role of orthophosphate and other factors in the regulation of starch formation in leaves and isolated chloroplasts. Plant Physiol. 1977 Jun;59(6):1146–1155.[PMC free article] [PubMed] [Google Scholar]
- Jacobus WE, Moreadith RW, Vandegaer KM. Mitochondrial respiratory control. Evidence against the regulation of respiration by extramitochondrial phosphorylation potentials or by [ATP]/[ADP] ratios. J Biol Chem. 1982 Mar 10;257(5):2397–2402. [PubMed] [Google Scholar]
- Kaiser WM, Bassham JA. Light-Dark Regulation of Starch Metabolism in Chloroplasts: II. Effect of Chloroplastic Metabolite Levels on the Formation of ADP-Glucose by Chloroplast Extracts. Plant Physiol. 1979 Jan;63(1):109–113.[PMC free article] [PubMed] [Google Scholar]
- Martin JB, Bligny R, Rebeille F, Douce R, Leguay JJ, Mathieu Y, Guern J. A P Nuclear Magnetic Resonance Study of Intracellular pH of Plant Cells Cultivated in Liquid Medium. Plant Physiol. 1982 Oct;70(4):1156–1161.[PMC free article] [PubMed] [Google Scholar]
- Nishimura M, Beevers H. Hydrolases in vacuoles from castor bean endosperm. Plant Physiol. 1978 Jul;62(1):44–48.[PMC free article] [PubMed] [Google Scholar]
- Rebeille F, Bligny R, Douce R. Regulation of Pi uptake by Acer pseudoplatanus cells. Arch Biochem Biophys. 1982 Dec;219(2):371–378. [PubMed] [Google Scholar]
- Rebeille F, Bligny R, Martin JB, Douce R. Relationship between the cytoplasm and the vacuole phosphate pool in Acer pseudoplatanus cells. Arch Biochem Biophys. 1983 Aug;225(1):143–148. [PubMed] [Google Scholar]
- Stitt M, Heldt HW. Physiological rates of starch breakdown in isolated intact spinach chloroplasts. Plant Physiol. 1981 Sep;68(3):755–761.[PMC free article] [PubMed] [Google Scholar]
- TAUSSKY HH, SHORR E. A microcolorimetric method for the determination of inorganic phosphorus. J Biol Chem. 1953 Jun;202(2):675–685. [PubMed] [Google Scholar]