Concentrations of glucose, sucrose, soluble reducing sugars, starch and total non-structural carbohydrate were determined during propagation of cuttings from sexually mature Pinus banksiana Lamb. trees. Such cuttings rarely initiate adventitious roots whatever the method or duration of propagation. Terminals, needles, and upper and basal stem segments of cuttings were analyzed at Day 0 and every 2 days for 18 days. Comparison of the results with those of earlier studies with cuttings of P. banksiana seedlings, which root readily, indicated pronounced differences in carbohydrate concentrations and partitioning between the two types of cutting. Compared with those from seedlings, cuttings from sexually mature trees exhibited: (1) more total non-structural carbohydrate in each tissue at Day 0; (2) decreasing rather than increasing total carbohydrate (mainly starch) concentrations in each tissue during propagation; (3) different carbohydrate concentration ratios in each tissue during propagation; and (4) higher sucrose concentrations in terminals during propagation, relative to concentrations at Day 0. Cuttings from sexually mature trees also differed from cuttings of seedlings in having a much lower rate of dry matter accumulation during propagation. These findings suggest that the poor rooting ability of cuttings from sexually mature P. banksiana is not attributable to a lack of total carbohydrate, but that the rooting abilities of cuttings from seedlings and from sexually mature trees differ because of differences between the two types of cutting in rates of net photosynthesis and starch metabolism. The difference in starch metabolism becomes apparent during the first 2 days of propagation.