In this study, the genotypic and environmental effects for bioactive compounds in strawberries were partitioned. 13 strawberry genotypes from diverse breeding programs were selected. The genotypes were grown in three growing conditions: greenhouse (GH), plastic tunnel (PT) and open-field (OF) for two growing seasons. The results indicated that the genotypes were significantly different for most of the characteristics tested except the ferric reducing ability assay (FRAP) and Trolox-equivalent antioxidant capacity assay (TEAC) in the second growing season, while the growing conditions were only significant for total phenolic content (TPC) and fructose and total sugar content in the first growing season. Genotype had 71% and 72% of the total variance for total monomeric anthocyanin contents (TMA), while it had only 12% and 13% of the variance for TPC in the first and second year of the experiment. Genotype effect was larger than that from the growing conditions for most of the bioactive component variables in the experiment indicated that breeding for bioactive components may be successful.