1-substituted aminomethyl-3-cyclohexylthiosemicarbazone-2-indolinones (I) were tested for their antibacterial activity against Bacillus pumilis, Bacillus brevis and Bacillus megaterium and antifungal activity against Aspergillus flavus, Aspergillus fumigatus and Aspergillus niger. The majority of the compounds were found to exhibit promising antibacterial and antifungal activities. These compounds were also screened for their antiviral action against tobacco mosaic virus in Nicotiana glutinosa plants in vivo as well as in vitro. Most of the compounds had shown significant antiviral activities both in vivo and in vitro.