Effects of copper-smelter-related deposition of heavy metals in the soil on carbohydrate metabolism of fine roots of the native European black poplar were investigated in spring and autumn. Total soluble non-structural carbohydrates in fine roots from trees growing in the polluted habitat were lower than in a control site, but this was directly associated only with a lower raffinose concentration. Neither glucose nor fructose concentrations differed significantly between polluted and unpolluted sites. In contrast, the galactose concentration was higher in the presence of heavy metals, especially in autumn. Also the stachyose concentration was higher in the polluted site, but only in autumn, suggesting it could be an alternative way of detoxification of galactose. No difference between control and polluted stands was observed in sucrose concentration. However, estimates of sucrolytic activity revealed markedly higher activities of sucrose synthase (SuSy), soluble acid (AI) and neutral (NI) invertases in the polluted stand than in the control. In contrast, the estimated glycolytic enzyme activities were not affected by the presence of heavy metals in soil.