The present research is preliminary biological screening of Euphorbia helioscopia L. (Euphorbiaceae). Dichloromethane and methanol extracts of the aerial parts of the plant were investigated for their antioxidant, antifungal, antibacterial and phytotoxic activities. Dichloromethane extract exhibited significant activity against Fusarium solani with 90% Inhibition, where as the same extract also showed non-significant activity against Salmonella typhi and Bacillus subtilis. Methanolic extract has promising radical-scavenging activity in this assay. Both the extracts have non-significant phytotoxicity against lemna minor.