Cloud-Clone Corp.
SEB514Hu
$466.00
48T
| 48T - | $466.00 | |
| 96T - | $665.00 | |
| 96T*5 - | $2,993.00 | |
| 96T*10 - | $5,653.00 | |
| 96T*100 - | $46,550.00 |
ELISA Kit for Interleukin 1 Receptor Associated Kinase 1 (IRAK1)
Reactivity:Human
Application:Enzyme-linked immunosorbent assay for Antigen Detection
Test method:Double-antibody Sandwich
Shelf life:Signal transduction
Storage conditions:Enzyme & Kinase
Delivery conditions:Tumor immunity
Usage:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Receptor Associated Kinase 1 (IRAK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 1 Receptor Associated Kinase 1 (IRAK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Receptor Associated Kinase 1 (IRAK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 1 Receptor Associated Kinase 1 (IRAK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sensitivity:The minimum detectable dose of this kit is typically less than 0.116ng/mL
Sample type:
tissue homogenates
cell lysates and other biological fluids
Assay length:3h
Specificity:
This assay has high sensitivity and excellent specificity for detection of Interleukin 1 Receptor Associated Kinase 1 (IRAK1).
No significant cross-reactivity or interference between Interleukin 1 Receptor Associated Kinase 1 (IRAK1) and analogues was observed.
Precision:
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 1 Receptor Associated Kinase 1 (IRAK1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 1 Receptor Associated Kinase 1 (IRAK1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability:
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Procedure:
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
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