Name: Phospho-Smad2 (Ser250) Antibody
Brand: Affinity Biosciences
Price: 280 USD

AF3450

$280.00

100μl

	100μl -	$280.00
	200μl -	$350.00

RIDACOM Ltd. hotline:

+3592 955 99 98

info@ridacom.com

Phospho-Smad2 (Ser250) Antibody

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Western blot analysis of Phospho-Smad2 (Ser250) Antibody expression in PMA treated 293 cells...

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AF3450 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton...

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ELISA analysis of AF3450 showing specificity to Phospho-Smad2 (Ser250) peptide. Peptides concentration:...

Antigen:

Mothers against decapentaplegic homolog 2

Synonyms: JV18-1; Mad-related protein 2; SMAD family member 2; SMAD2_HUMAN; Q15796

BioSeek|Uniprot

Host:Rabbit

Reactivity:Human; Mouse; Rat

Application:Western Blotting; ELISA

Isotype:IgG

Clonality:Polyclonal

Aliases:Drosophila, homolog of, MADR2; hMAD-2; HsMAD2; JV18; JV18-1; JV181; MAD; MAD homolog 2; MAD Related Protein 2; Mad-related protein 2; MADH2; MADR2; MGC22139; MGC34440; Mother against DPP homolog 2; Mothers against decapentaplegic homolog 2; Mothers against decapentaplegic, Drosophila, homolog of, 2; Mothers against DPP homolog 2; OTTHUMP00000163489; Sma and Mad related protein 2; Sma- and Mad-related protein 2 MAD; SMAD 2; SMAD family member 2; SMAD, mothers against DPP homolog 2; SMAD2; SMAD2_HUMAN

Description:

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

Purification:The antibody is from purified?rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Concentration:1mg/ml

Buffer form:Liquid

Storage buffer:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 ¡ãC.Stable for 12 months from date of receipt

Specificity:

Phospho-Smad2 (Ser250) Antibody detects endogenous levels of Smad2 only when phosphorylated at Serine 250.

Storage conditions:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Product specifications