Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner (PubMed:20074560). Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.
Purification:The antibody is from purified?rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Concentration:1mg/ml
Buffer form:Liquid
Storage buffer:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 ¡ãC.Stable for 12 months from date of receipt
Specificity:
Phospho-Estrogen Receptor- beta (Ser105) Antibody detects endogenous levels of Estrogen Receptor- beta only when phosphorylated at Serine 105.
Storage conditions:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.