PurKine™ Protein AT Purification system is effective for affinity purification of monoclonal antibodies at process scale. Protein At is an alkali-resistant protein A (Alkaline tolerate) based on the native protein A though biotechnology mutation. This ligand provides greater stability than conventional protein A, and cleaning can be performed with cost-effective reagents such as sodium hydroxide eliminating the need for expensive and hazardous cleaning agents. The portfolio allow optimization of process for maximum protein yield, stability and solubility. Tests also confirm that no decrease in performance occurs after at least five repeated uses. The PurKine™ Protein AT Purification Resin is also available as prepacked spin column and kit formats.