Abbkine Scientific Co., Ltd.
KTE70537
$345.00
48T
| 48T - | $345.00 | |
| 96T - | $650.00 | |
| 96T*5 - | $2,925.00 | |
| 96T*50 - | $26,000.00 |
Mouse Angiotensin I converting enzyme (ACE) ELISA Kit
Fig.1. Mouse Angiotensin I converting enzyme (ACE) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples....Antigen:
Angiotensin-converting enzyme
Synonyms: Dipeptidyl carboxypeptidase I; Kininase II; ACE_MOUSE; P09470; P22967; Q6GTS2
Description:
Angiotensin I-converting enzyme (ACE, EC 3.4.15.1), an exopeptidase, is a circulating enzyme that participates in the body's renin-angiotensin system (RAS), which mediates extracellular volume (i.e. that of the blood plasma, lymph and interstitial fluid), and arterial vasoconstriction. It is secreted by pulmonary and renal endothelial cells and catalyzes the conversion of decapeptide angiotensin I to octapeptide angiotensin II. ACE Ubiquitously expressed, with highest levels in lung, kidney, heart, gastrointestinal system and prostate. The testis-specific isoforn is expressed in spermatocytes, adult testis. ACE can Convert angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin.
Test method:Colorimetric
Precautions:
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Alternative:
ACE
ACE1
CD143
DCP
DCP1
MGC26566
MVCD3
CD143 antigen
angiotensin I converting enzyme 1
angiotensin converting enzyme
somatic isoform
carboxycathepsin
dipeptidyl carboxypeptidase 1
kininase
pep
Storage conditions:The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Delivery conditions:Gel pack with blue ice.
Usage:
• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Sensitivity:0.135 ng/mL
Sample type:
Cell culture supernatants
Serum
Plasma
Other biological fluids
Assay length:Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components:
• Mouse Angiotensin I converting enzyme microplate
• Mouse Angiotensin I converting enzyme standard
• Mouse Angiotensin I converting enzyme detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Calibration range:0.312 - 20ng/mL