Abbkine Scientific Co., Ltd.
KTE60250
$280.00
48T
| 48T - | $280.00 | |
| 96T - | $480.00 | |
| 96T*5 - | $2,160.00 | |
| 96T*50 - | $20,160.00 |
Human Thymosin beta-4 (TMSB4X) ELISA Kit
Fig.1. Human Thymosin beta-4 (TMSB4X) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples....Antigen:
Thymosin beta-4
Synonyms: Fx; TYB4_HUMAN; P01253; P01254; P62328; Q546P5; Q63576; Q9UE55
Description:
The predominant form of thymosin, thymosin beta4, is a member of a highly conserved family of actin monomer-sequestering proteins. Beta-thymosins are the primary regulators of unpolymerized actin, and are essential for maintaining the small cytoplasmic pool of free G-actin monomers required for rapid filament elongation and allowing for the flux of monomers between the thymosin-bound pool and F-actin. Thymosin beta4 sequesters actin, holding it in a form that is unable to polymerize. Due to its profusion in the cytosol and its ability to bind ATP G-actin but not F-actin, thymosin beta4 is regarded as the principal actin-sequestering protein. Thymosin beta4 binds ATP G- monomeric actin in a 11 complex where G-actin cannot polymerize.
Test method:Colorimetric
Precautions:
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Alternative:
TMSB4X
FX
PTMB4
TB4X
TMSB4
OTTHUMP00000022925
OTTHUMP00000022927
OTTHUMP00000022928
prothymosin beta-4
thymosin beta-4
thymosin
beta 4
thymosin
beta 4
X chromosome
Storage conditions:The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Delivery conditions:Gel pack with blue ice.
Usage:
• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Sensitivity:69.27 pg/mL
Sample type:
Cell culture supernatants
Serum
Plasma
Other biological fluids
Assay length:Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components:
• Human Thymosin beta-4 microplate
• Human Thymosin beta-4 standard
• Human Thymosin beta-4 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Calibration range:56.25 - 3000pg/mL