Abbkine Scientific Co., Ltd.
KTE60558
$280.00
48T
| 48T - | $280.00 | |
| 96T - | $480.00 | |
| 96T*5 - | $2,160.00 | |
| 96T*50 - | $20,160.00 |
Human Sorbitol dehydrogenase (SDH) ELISA Kit
Fig.1. Human Sorbitol dehydrogenase (SDH) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples....Antigen:
Sorbitol dehydrogenase
Synonyms: L-iditol 2-dehydrogenase; DHSO_HUMAN; B2R655; B7Z3A6; J3JZZ5; Q00796; Q16682; Q9UMD6
Description:
Sorbitol dehydrogenase (SORD; EC 1.1.1.14) catalyzes the interconversion of polyols and their corresponding ketoses, and together with aldose reductase (ALDR1; MIM 103880), makes up the sorbitol pathway that is believed to play an important role in the development of diabetic complications (summarized by Carr and Markham, 1995 [PubMed 8535074]). The first reaction of the pathway (also called the polyol pathway) is the reduction of glucose to sorbitol by ALDR1 with NADPH as the cofactor. SORD then oxidizes the sorbitol to fructose using NAD(+) cofactor.
Test method:Colorimetric
Precautions:
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Alternative:
SORD
SORD1
Storage conditions:The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Delivery conditions:Gel pack with blue ice.
Usage:
• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Sensitivity:81.32 pg/mL
Sample type:
Cell culture supernatants
Serum
Plasma
Other biological fluids
Assay length:Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components:
• Human Sorbitol dehydrogenase microplate
• Human Sorbitol dehydrogenase standard
• Human Sorbitol dehydrogenase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Calibration range:56.25 - 3000pg/mL