Abbkine Scientific Co., Ltd.
KTE60641
$260.00
48T
| 48T - | $260.00 | |
| 96T - | $450.00 | |
| 96T*5 - | $2,025.00 | |
| 96T*50 - | $18,900.00 |
Human RAC-alpha serine/threonine-protein kinase (AKT1) ELISA Kit
Fig.1. Human RAC-alpha serine/threonine-protein kinase (AKT1) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples....Antigen:
RAC-alpha serine/threonine-protein kinase
Synonyms: Protein kinase B; Protein kinase B alpha; Proto-oncogene c-Akt; RAC-PK-alpha; AKT1_HUMAN; B2RAM5; B7Z5R1; P31749; Q9BWB6
Description:
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.
Test method:Colorimetric
Precautions:
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Alternative:
AKT1
AKT
MGC99656
PKB
PKB-ALPHA
PRKBA
RAC
RAC-ALPHA
AKT1 kinase
RAC-alpha serine/threonine-protein kinase
protein kinase B
rac protein kinase alpha
Storage conditions:The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Delivery conditions:Gel pack with blue ice.
Usage:
• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Sensitivity:0.054 ng/mL
Sample type:
Cell culture supernatants
Serum
Plasma
Other biological fluids
Assay length:Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components:
• Human RAC-alpha serine/threonine-protein kinase microplate
• Human RAC-alpha serine/threonine-protein kinase standard
• Human RAC-alpha serine/threonine-protein kinase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Calibration range:0.156 - 10ng/mL