Abbkine Scientific Co., Ltd.
KTE60811
$280.00
48T
| 48T - | $280.00 | |
| 96T - | $480.00 | |
| 96T*5 - | $2,160.00 | |
| 96T*50 - | $20,160.00 |
Human Nuclear factor-KB p65 (NF-KB p65) ELISA Kit
Fig. Abbkine ELISA kit is series of ELISA to quantitate specific protein in samples....Antigen:
Transcription factor p65
Synonyms: Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; TF65_HUMAN; Q04206; Q6GTV1; Q6SLK1
Description:
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of RELA. Four transcript variants encoding different isoforms have been found for RELA.
Test method:Colorimetric
Precautions:
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Alternative:
RELA
MGC131774
NFKB3
p65
nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene
Storage conditions:The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Delivery conditions:Gel pack with blue ice.
Usage:
• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Sensitivity:11.75 pg/mL
Sample type:
Cell culture supernatants
Serum
Plasma
Other biological fluids
Assay length:Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components:
• Human Nuclear factor-KB p65 microplate
• Human Nuclear factor-KB p65 standard
• Human Nuclear factor-KB p65 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Calibration range:47 - 3000pg/mL