Abbkine Scientific Co., Ltd.
PRP100077
$370.00
20µg
| 20µg - | $370.00 | |
| 100µg - | $940.00 |
Human Noggin protein
Fig.SDS-PAGE analysis of Human Noggin protein.Aliases:Noggin; SYM1; SYNS1; Noggin; NOG
Description:
Noggin is a secreted protein involved at multiple stages of vertebrate embryonic development including neural induction and is known to exert its effects by inhibiting the bone morphogenetic protein (BMP)-signaling pathway. It binds several BMPs with very high (picomolar) affinities, with a marked preference for BMP2 and BMP4 over BMP7. By binding tightly to BMPs, Noggin prevents BMPs from binding their receptors. Noggin binds the bone morphogenetic proteins (BMP) such as BMP-4 and BMP-7, and inhibits BMP signaling by blocking the molecular interfaces of the binding epitopes for both type I and type II receptors. Interaction of BMP and its antagonist Noggin governs various developmental and cellular processes, including embryonic dorsal-ventral axis, induction of neural tissue, formation of joints in the skeletal system and neurogenesis in the adult brain. Noggin plays a key role in neural induction by inhibiting BMP4, along with other TGF-β signaling inhibitors such as chordin and follistatin. Mouse knockout experiments have demonstrated that noggin also plays a crucial role in bone development, joint formation, and neural tube fusion.
Preparation method:Human; Cells
Activity:
Measured by its ability to inhibit BMP4-induced alkaline phosphatase production by MC3T3-E1 cells.
The ED50 for this effect is typically 0.18-0.7 ug/mL in the presence of 50 ng/mL of hBMP4.
Formulation:Lyophilized from sterile PBS, pH 7.4.
Storage conditions:Lyophilized Human Noggin protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Delivery conditions:The product is shipped at ambient temperature.
Usage:
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human Noggin protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Purity:> 85 % as determined by SDS-PAGE.
Molecular weight:23.7
Sequence:Amino acid sequence derived from human NOG (NP_005441.1) (Met1-Cys232) was expressed with five amino acids (DDDDK) at the C-terminus.
Protein length:The recombinant human NOG consists 211 amino acids and predicts a molecular mass of 23.7 kDa.
Precautions:
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Others:The Endotoxin level is less than 1.0 EU per μg protein as determined by the LAL method.