Abbkine Scientific Co., Ltd.
KTE61732
$260.00
48T
| 48T - | $260.00 | |
| 96T - | $450.00 | |
| 96T*5 - | $2,025.00 | |
| 96T*50 - | $18,900.00 |
Human Mitogen-activated protein kinase 3 (MAPK3) ELISA Kit
Fig.1. Human Mitogen-activated protein kinase 3 (MAPK3) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples....Antigen:
Mitogen-activated protein kinase 3
Synonyms: ERT2; Extracellular signal-regulated kinase 1; Insulin-stimulated MAP2 kinase; MAP kinase isoform p44; Microtubule-associated protein 2 kinase; p44-ERK1; MK03_HUMAN; A8CZ58; B0LPG3; P27361; Q8NHX1
Description:
Mitogen-activated protein kinase 3 is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.
Test method:Colorimetric
Precautions:
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Alternative:
MAPK3
ERK1
HS44KDAP
HUMKER1A
MGC20180
P44ERK1
P44MAPK
PRKM3
OTTHUMP00000174538
extracellular signal-regulated kinase 1
extracellular signal-related kinase 1
Storage conditions:The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Delivery conditions:Gel pack with blue ice.
Usage:
• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Sensitivity:0.118 ng/mL
Sample type:
Cell culture supernatants
Serum
Plasma
Other biological fluids
Assay length:Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components:
• Human Mitogen-activated protein kinase 3 microplate
• Human Mitogen-activated protein kinase 3 standard
• Human Mitogen-activated protein kinase 3 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Calibration range:0.312 - 20ng/mL