Abbkine Scientific Co., Ltd.
KTE60567
$280.00
48T
| 48T - | $280.00 | |
| 96T - | $480.00 | |
| 96T*5 - | $2,160.00 | |
| 96T*50 - | $20,160.00 |
Human Acid sphingomyelinase (ASM) ELISA Kit
Fig.1. Human Acid sphingomyelinase (ASM) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples....Antigen:
Sphingomyelin phosphodiesterase
Synonyms: Acid sphingomyelinase; ASM_HUMAN; A8K8M3; E9PKS3; P17405; P17406; Q13811; Q16837; Q16841
Description:
Sphingomyelin phosphodiesterase or simply Sphingomyelinase (SMase) is a hydrolase enzyme that is involved in sphingolipid metabolism reactions. SMase is a member of the DNase I superfamily of enzymes and is responsible for breaking sphingomyelin (SM) down into phosphocholine and ceramide. The activation of SMase has been suggested as a major route for the production of ceramide in response to cellular stresses. Sphingomyelin phosphodiesterase 1, acid lysosomal (acid sphingomyelinase), also known as SMPD1, is a human sphingomyelin phosphodiesterase. Defects in SMPD1 gene cause Niemann-Pick disease, SMPD1-associated.
Test method:Colorimetric
Precautions:
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Alternative:
SMPD1
ASM
NPD
acid sphingomyelinase
Storage conditions:The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Delivery conditions:Gel pack with blue ice.
Usage:
• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Sensitivity:0.057 ng/mL
Sample type:
Cell culture supernatants
Serum
Plasma
Other biological fluids
Assay length:Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components:
• Human Acid sphingomyelinase microplate
• Human Acid sphingomyelinase standard
• Human Acid sphingomyelinase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Calibration range:0.156 - 10ng/mL