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$280.00
48T
48T -$280.00
96T -$480.00
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Canine Golgi membrane protein 1 (GOLM1) ELISA Kit
Antigen:
Golgi membrane protein 1
Synonyms: Golgi membrane protein GP73; Golgi phosphoprotein 2; GOLM1_HUMAN; Q6IAF4; Q8NBJ4; Q9NRB9
Description:
Golgi protein-73 (GP73), a resident Golgi glycoprotein, is up-regulated in hepatocellular carcinoma (HCC). GP73 is a novel serum marker for HCC.The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. 1 is a type II Golgi transmembrane protein. It processes protein synthesized in the rough endoplasmic reticulum and assists in the transport of protein cargo through the Golgi apparatus. The expression of this encoded protein has been observed to be upregulated in response to viral infection. Two alternatively spliced transcript variants encoding the same protein have been described for this gene.It is overexpressed in prostate cancer.
Test method:Colorimetric
Precautions:
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Alternative:
GOLM1
Storage conditions:The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Delivery conditions:Gel pack with blue ice.
Usage:
• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Sensitivity:49.46 pg/mL
Sample type:
Cell culture supernatants
Serum
Plasma
Other biological fluids
Assay length:Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components:
• Canine Golgi membrane protein 1 microplate
• Canine Golgi membrane protein 1 standard
• Canine Golgi membrane protein 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Calibration range:56.25 - 3000pg/mL