Hepatocyte growth factor receptor (MET) gene encodes a member of the receptor tyrosine kinase family of proteins and the product of the proto-oncogene MET. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that are linked via disulfide bonds to form the mature receptor. Further processing of the beta subunit results in the formation of the M10 peptide, which has been shown to reduce lung fibrosis. Binding of its ligand, hepatocyte growth factor, induces dimerization and activation of the receptor, which plays a role in cellular survival, embryogenesis, and cellular migration and invasion. Mutations in this gene are associated with papillary renal cell carcinoma, hepatocellular carcinoma, and various head and neck cancers. Amplification and overexpression of this gene are also associated with multiple human cancers.

Test method:Colorimetric

Precautions:

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Alternative:

MET

AUTS9

HGFR

RCCP2

c-Met

HGF receptor

SF receptor

met proto-oncogene

met proto-oncogene tyrosine kinase

oncogene MET

scatter factor receptor

Storage conditions:The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Delivery conditions:Gel pack with blue ice.

Usage:

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.

• Allow all reagents to warm to room temperature for at least 30 minutes before opening.

• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.

• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.

• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.

• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Sensitivity:55.05 pg/mL

Sample type:

Cell culture supernatants

Serum

Plasma

Other biological fluids

Assay length:Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit components:

• Bovine Hepatocyte growth factor receptor microplate

• Bovine Hepatocyte growth factor receptor standard

• Bovine Hepatocyte growth factor receptor detect antibody

• Streptavidin-HRP

• Standard diluent

• Assay buffer

• HRP substrate

• Stop solution

• Wash buffer

• Plate covers

Calibration range:56.25 - 3000pg/mL