Hi-SYBr Master Mix contains Hi-Temp DNA Polymerase for hot-start PCR, to improve specificity and sensitivity of the PCR by minimizing the formation of non-specific amplification products. The enzyme is active only at high temperatures, where primers no longer bind non-specifically.

Concentration : 5units/µl Supplied with: 10X Hibuffer A with KCl, 10X Hibuffer S with Ammonium sulphate. Ammonium sulphate allows for PCR at wide range of Mg2+ concentrations and decrease unspecific priming] Hi-Proof DNA Polymerase is an extremely thermostable polymerase from the hyperthermophilicarchaeum Pyrococcusfuriosus. Hi-Proof DNA Polymerase catalyzes the polymerization of nucleotides into duplex DNA in the 5’ to 3’ direction in the presence of Mg2+. It exhibits the 3’ to 5’ exonuclease (Proofreading) activity that enables the polymerase to correct nucleotide incorporation errors resulting in over 10-fold higher fidelity than possible with Taq DNA Polymerases. It has no 5’>3’ exonuclease activity.

Concentration : 5units/µl Supplied with: 10X Hibuffer A with KCl, 10X Hibuffer S with Ammonium sulphate. Ammonium sulphate allows for PCR at wide range of Mg2+ concentrations and decrease unspecific priming] Hi-Temp DNA Polymerase is a complex of specific anti-Taq monoclonal antibody with best quality thermostableTaq DNA Polymerase for automatic “hot start” amplification, resulting in greatly enhanced amplification specificity, sensitivity and yield. Hi-Temp DNA Polymerase catalyses the polymerization of nucleotides into duplex DNA in the 5’-3’ direction in the presence of Mg2+ and has the 5’-3’ exonuclease activity.

dNTP Mix, containing dATP, dCTP, dGTP, dTTP are supplied as 100 mM aqueous solution at pH 7.0. dNTPs are available individually as nucleotide mixes at convenient concentrations for direct use in PCR, reverse transcription, labeling and other applications • Greater than 99% purity confirmed by HPLC • Free of Human and E.coli DNA • Highly stable: - stable after multiple freeze-thaw cycles - 85-90% of dNTPs remain in triphosphate form after 30 cycles of PCR

AMV Reverse Transcriptase (Avian Myeloblastosis Virus Reverse Transcriptase) is an RNA-dependent DNA polymerase with a molecular weight of 157,000 daltons. This enzyme can synthesize a complementary DNA strand initiating from a primer using either RNA (cDNA synthesis) or single-stranded DNA as template.