Cloud-Clone Corp.
MEB526Ge
$559.00
48T
| 48T - | $559.00 | |
| 96T - | $798.00 | |
| 96T*5 - | $3,591.00 | |
| 96T*10 - | $6,783.00 | |
| 96T*100 - | $55,860.00 |
Mini Samples ELISA Kit for Lipopolysaccharide (LPS)
Antigen:
Reactivity:General
Application:Enzyme-linked immunosorbent assay for Antigen Detection
Test method:Double-antibody Sandwich
Shelf life:Endotoxin
Storage conditions:Lipopolysaccharide
Usage:
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Mini Samples Lipopolysaccharide (LPS) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Mini Samples Lipopolysaccharide (LPS) and unlabeled Mini Samples Lipopolysaccharide (LPS) (Standards or samples) with the pre-coated antibody specific to Mini Samples Lipopolysaccharide (LPS). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Mini Samples Lipopolysaccharide (LPS) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Mini Samples Lipopolysaccharide (LPS) in the sample.
Sensitivity:The minimum detectable dose of this kit is typically less than 4.45ng/mL
Sample type:
serum
plasma
tissue homogenates
cell lysates
cell culture supernates and other biological fluids
Assay length:3h
Specificity:
This assay has high sensitivity and excellent specificity for detection of Mini Samples Lipopolysaccharide (LPS).
No significant cross-reactivity or interference between Mini Samples Lipopolysaccharide (LPS) and analogues was observed.
Precision:
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Lipopolysaccharide (LPS) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Lipopolysaccharide (LPS) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability:
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
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