Cloud-Clone Corp.
MEA639Mu
$545.00
48T
| 48T - | $545.00 | |
| 96T - | $778.00 | |
| 96T*5 - | $3,501.00 | |
| 96T*10 - | $6,613.00 | |
| 96T*100 - | $54,460.00 |
Mini Samples ELISA Kit for Cross Linked N-Telopeptide Of Type I Collagen (NTXI)
Reactivity:Mouse
Application:Enzyme-linked immunosorbent assay for Antigen Detection
Test method:Double-antibody Sandwich
Shelf life:Bone metabolism
Usage:
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Mini Samples Cross Linked N-Telopeptide Of Type I Collagen (NTXI) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Mini Samples Cross Linked N-Telopeptide Of Type I Collagen (NTXI) and unlabeled Mini Samples Cross Linked N-Telopeptide Of Type I Collagen (NTXI) (Standards or samples) with the pre-coated antibody specific to Mini Samples Cross Linked N-Telopeptide Of Type I Collagen (NTXI). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Mini Samples Cross Linked N-Telopeptide Of Type I Collagen (NTXI) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Mini Samples Cross Linked N-Telopeptide Of Type I Collagen (NTXI) in the sample.
Sensitivity:The minimum detectable dose of this kit is typically less than 0.89ng/mL
Sample type:
serum
plasma
urine and other biological fluids
Assay length:3h
Specificity:
This assay has high sensitivity and excellent specificity for detection of Mini Samples Cross Linked N-Telopeptide Of Type I Collagen (NTXI).
No significant cross-reactivity or interference between Mini Samples Cross Linked N-Telopeptide Of Type I Collagen (NTXI) and analogues was observed.
Precision:
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Cross Linked N-Telopeptide Of Type I Collagen (NTXI) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Cross Linked N-Telopeptide Of Type I Collagen (NTXI) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability:
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Procedure:
1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C;
3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 20µL Stop Solution. Read at 450nm immediately.
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