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Instant ELISA Kit for Estrone (E1)
Synonyms: Oestrone
Application:Enzyme-linked immunosorbent assay for Antigen Detection
Research area:Metabolic pathway
Test method:Competitive Inhibition
Shelf life:Reproductive science
Storage conditions:Genetic science
Delivery conditions:Hormone metabolism
The test principle applied in this kit is enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Instant Estrone (E1). Standards or samples and HRP-labeled detection antibody specific to Instant Estrone (E1) (Detection Reagent A) are then added to the appropriate microtiter plate wells. Next, TMB substrate solution is added, only those wells that contain Instant Estrone (E1), and HRP-labeled detection antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Instant Estrone (E1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sensitivity:The minimum detectable dose of this kit is typically less than
Assay length:1h, 10min
This assay has high sensitivity and excellent specificity for detection of Instant Estrone (E1).
No significant cross-reactivity or interference between Instant Estrone (E1) and analogues was observed.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Instant Estrone (E1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Instant Estrone (E1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample and 50µL Detection Reagent A to each well. Incubate 1 hours at 37°C;
3. Aspirate and wash 3 times;
4. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
5. Add 50µL Stop Solution. Read at 450nm immediately.