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$836.00
48T
48T -$836.00
96T -$1,194.00
96T*5 -$5,373.00
96T*10 -$10,149.00
96T*100 -$83,580.00
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Instant ELISA Kit for Androsterone (ADT)
Antigen:
17-KS
Synonyms: 17-Ketosteroid
Reactivity:General
Application:Enzyme-linked immunosorbent assay for Antigen Detection
Test method:Competitive Inhibition
Delivery conditions:8 months
Usage:
The test principle applied in this kit is enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Instant Androsterone (ADT). Standards or samples and HRP-labeled detection antibody specific to Instant Androsterone (ADT) (Detection Reagent A) are then added to the appropriate microtiter plate wells. Next, TMB substrate solution is added, only those wells that contain Instant Androsterone (ADT), and HRP-labeled detection antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Instant Androsterone (ADT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sensitivity:The minimum detectable dose of this kit is typically less than
Assay length:1h, 10min
Specificity:
This assay has high sensitivity and excellent specificity for detection of Instant Androsterone (ADT).
No significant cross-reactivity or interference between Instant Androsterone (ADT) and analogues was observed.
Precision:
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Instant Androsterone (ADT) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Instant Androsterone (ADT) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability:
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Procedure:
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample and 50µL Detection Reagent A to each well. Incubate 1 hours at 37°C;
3. Aspirate and wash 3 times;
4. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
5. Add 50µL Stop Solution. Read at 450nm immediately.