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ELISA Kit for Progesterone (PG)
Synonyms: P4; Pregn-4-Ene-3,20-Dione
Application:Enzyme-linked immunosorbent assay for Antigen Detection
Research area:Endocrinology
Test method:Competitive Inhibition
Shelf life:Hormone metabolism
Delivery conditions:Prevention of preterm birth by progestational agents: what are the molecular mechanisms? ;The role of Sep (O-phosphoserine) tRNA: Sec (selenocysteine) synthase (SEPSECS) in proliferation, apoptosis and hormone secretion of trophoblast cells;Neutrophil gene dynamics and plasma cytokine levels in dairy cattle during peri-implantation period;The Reproductive Toxicity of CdSe/ZnS Quantum Dots on the in vivo Ovarian Function and in vitro Fertilization;Molecular characterization of insulin resistance and glycolytic metabolism in the rat uterus;Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome.;Exposure of pregnant mice to perfluorobutanesulfonate causes hypothyroxinemia and developmental abnormalities in female offspring;Middle-Aged Diabetic Females and Males Present Distinct Susceptibility to Alzheimer Disease-like Pathology.;Exposure of pregnant mice to triclosan impairs placental development and nutrient transport.;Acute administration of oestradiol or progesterone in a spinal cord ischaemia–reperfusion model in rats;Exposure of Pregnant Mice to Triclosan Causes Insulin Resistance via Thyroxine Reduction;The anorectic agent, lorcaserin, disturbs estrous cyclicity and produces endometrial hyperplasia without affecting ovarian population in female rats;Progesterone attenuates hypertension and autoantibody levels to the angiotensin II type 1 receptor in response to elevated cadmium during pregnancy;The biocompatibility studies of polymer dots on pregnant mice and fetuses;Beneficial effects of Heqi san on rat model of polycystic ovary syndrome through the PI3K/AKT pathway;
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Progesterone (PG) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Progesterone (PG) and unlabeled Progesterone (PG) (Standards or samples) with the pre-coated antibody specific to Progesterone (PG). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Progesterone (PG) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Progesterone (PG) in the sample.
Sensitivity:The minimum detectable dose of this kit is typically less than 0.47ng/mL
Sample type:
plasma and other biological fluids
Assay length:2h
This assay has high sensitivity and excellent specificity for detection of Progesterone (PG).
No significant cross-reactivity or interference between Progesterone (PG) and analogues was observed.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Progesterone (PG) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Progesterone (PG) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
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