Cloud-Clone Corp.
CEA573Hu
$441.00
48T
| 48T - | $441.00 | |
| 96T - | $630.00 | |
| 96T*5 - | $2,835.00 | |
| 96T*10 - | $5,355.00 | |
| 96T*100 - | $44,100.00 |
ELISA Kit for Procollagen III N-Terminal Propeptide (PIIINP)
Results demonstration
Typical Standard Curve
Antigen:
Reactivity:Human
Application:Enzyme-linked immunosorbent assay for Antigen Detection
Test method:Competitive Inhibition
Shelf life:Metabolic pathway
Storage conditions:Hepatology
Delivery conditions:Bone metabolism
Usage:
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Procollagen III N-Terminal Propeptide (PIIINP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Procollagen III N-Terminal Propeptide (PIIINP) and unlabeled Procollagen III N-Terminal Propeptide (PIIINP) (Standards or samples) with the pre-coated antibody specific to Procollagen III N-Terminal Propeptide (PIIINP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Procollagen III N-Terminal Propeptide (PIIINP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Procollagen III N-Terminal Propeptide (PIIINP) in the sample.
Sensitivity:The minimum detectable dose of this kit is typically less than 2.13ng/mL
Sample type:
serum
plasma
tissue homogenates
cell lysates
cell culture supernates and other biological fluids
Assay length:2h
Specificity:
This assay has high sensitivity and excellent specificity for detection of Procollagen III N-Terminal Propeptide (PIIINP).
No significant cross-reactivity or interference between Procollagen III N-Terminal Propeptide (PIIINP) and analogues was observed.
Precision:
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Procollagen III N-Terminal Propeptide (PIIINP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Procollagen III N-Terminal Propeptide (PIIINP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability:
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Procedure:
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
Preparing document for printing...
0%