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$588.00
48T
48T -$588.00
96T -$840.00
96T*5 -$3,780.00
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CLIA Kit for C Reactive Protein (CRP)
Antigen:
PTX1
Synonyms: C-RP
Reactivity:Human
Application:Chemiluminescent immunoassay for Antigen Detection
Test method:Double-antibody Sandwich
Shelf life:C Reactive Protein
Storage conditions:Tumor immunity
Delivery conditions:Infection immunity
Usage:
The microplate provided in this kit has been pre-coated with an antibody specific to C Reactive Protein (CRP). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to C Reactive Protein (CRP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the C Reactive Protein (CRP) level in the sample or standard.;
Sensitivity:The minimum detectable dose of this kit is typically less than 5.1pg/mL
Sample type:
Serum
plasma
tissue homogenates
cell lysates
urine
cerebrospinal fluid
cell culture supernates and other biological fluids
Assay length:2h, 40min
Specificity:
This assay has high sensitivity and excellent specificity for detection of C Reactive Protein (CRP).
No significant cross-reactivity or interference between C Reactive Protein (CRP) and analogues was observed.
Precision:
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level C Reactive Protein (CRP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level C Reactive Protein (CRP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability:
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Procedure:
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.
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