Abbkine Scientific Co., Ltd.
KTE110017
$280.00
48T
| 48T - | $280.00 | |
| 96T - | $480.00 | |
| 96T*5 - | $2,160.00 | |
| 96T*50 - | $20,160.00 |
Sheep Prostaglandin G/H synthase 2 (PTGS2) ELISA Kit
Fig.1. Sheep Prostaglandin G/H synthase 2 (PTGS2) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples....Antigen:
Prostaglandin G/H synthase 2
Synonyms: Cyclooxygenase-2; PHS II; Prostaglandin H2 synthase 2; Prostaglandin-endoperoxide synthase 2; PGH2_SHEEP; P79208
Description:
Cyclooxygenase (COX) enzymes catalyze the synthesis of prostaglandins (PGs) from arachidonic acid. There are two isoforms of COX, COX-1 and COX-2. While COX-1 is expressed constitutively and appears to be responsible for the production of prostaglandins (PGs) that control normal physiologic functions, expression of COX-2 is induced by various inflammatory and mitogenic stimuli such as cytokines, growth factors or tumor promoters. Numerous experimental studies suggest a relationship between COX-2 expression and carcinogenesis. For example, increased amounts of COX-2 have been observed in breast cancers that overexpress HER-2/neu. Furthermore, treatment with selective inhibitors of COX-2 reduced the formation of tongue, esophagal, intestinal, breast, skin, lung, and bladder tumors in experimental animals.
Test method:Colorimetric
Precautions:
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Alternative:
PTGS2
COX-2
COX2
GRIPGHS
PGG/HS
PGHS-2
PHS-2
hCox-2
cyclooxygenase 2b
prostaglandin G/H synthase and cyclooxygenase
prostaglandin-endoperoxide synthase 2
Storage conditions:The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Delivery conditions:Gel pack with blue ice.
Usage:
• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Sensitivity:60.27 pg/mL
Sample type:
Cell culture supernatants
Serum
Plasma
Other biological fluids
Assay length:Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components:
• Sheep Prostaglandin G/H synthase 2 microplate
• Sheep Prostaglandin G/H synthase 2 standard
• Sheep Prostaglandin G/H synthase 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Calibration range:75 - 4800pg/mL