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$260.00
48T
48T -$260.00
96T -$450.00
96T*5 -$2,025.00
96T*50 -$18,900.00
RIDACOM Ltd. hotline:
Rat Solute carrier family 2, facilitated glucose transporter member 1 (SLC2A1) ELISA Kit
Antigen:
Solute carrier family 2, facilitated glucose transporter member 1
Synonyms: Glucose transporter type 1, erythrocyte/brain; GTR1_RABIT; P13355
Description:
GLUT1 facilitates the transport of glucose across the plasma membranes of mammalian cells.GLUT1 is responsible for the low-level of basal glucose uptake required to sustain respiration in all cells. Expression levels of GLUT1 in cell membranes are increased by reduced glucose levels and decreased by increased glucose levels. GLUT1 is also a major receptor for take-up of Vitamin C as well as glucose, especially in non vitamin C producing mammals as part of an adaptation to compensate by participating in a Vitamin C recycling process. In mammals that do produce Vitamin C, GLUT4 is often expressed instead of GLUT1.GLUT1 behaves as a Michaelis-Menten enzyme and contains 12 membrane-spanning alpha helices, each containing 20 amino acid residues.
Test method:Colorimetric
Precautions:
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Alternative:
SLC2A1
DYT17
DYT18
GLUT
GLUT1
MGC141895
MGC141896
PED
Storage conditions:The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Delivery conditions:Gel pack with blue ice.
Usage:
• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Sensitivity:0.48 ng/mL
Sample type:
Cell culture supernatants
Serum
Plasma
Other biological fluids
Assay length:Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components:
• Rat Solute carrier family 2, facilitated glucose transporter member 1 microplate
• Rat Solute carrier family 2, facilitated glucose transporter member 1 standard
• Rat Solute carrier family 2, facilitated glucose transporter member 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Calibration range:1.25 - 80ng/mL