Abbkine Scientific Co., Ltd.
KTE100863
$260.00
48T
| 48T - | $260.00 | |
| 96T - | $450.00 | |
| 96T*5 - | $2,025.00 | |
| 96T*50 - | $18,900.00 |
Rat Cyclooxygenase-2 (COX-2) ELISA Kit
Fig.1. Rat Cyclooxygenase-2 (COX-2) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples....Antigen:
Prostaglandin G/H synthase 2
Synonyms: Cyclooxygenase-2; PHS II; Prostaglandin H2 synthase 2; Prostaglandin-endoperoxide synthase 2; PGH2_RABIT; O02768
Description:
Cytochrome c oxidase subunit II is a human protein belongs to cytochrome c oxidase subunit II protein family. It is sometimes confused with the cyclooxygenase enzyme COX-2.Cytochrome c oxidase subunit II, abbreviated CoxII, is the second subunit of cytochrome c oxidase. Cytochrome c oxidase (EC 1.9.3.1) is an oligomeric enzymatic complex which is a component of the respiratory chain and is involved in the transfer of electrons from cytochrome c to oxygen. In eukaryotes this enzyme complex is located in the mitochondrial inner membrane; in aerobic prokaryotes it is found in the plasma membrane. The enzyme complex consists of 3-4 subunits (prokaryotes) to up to 13 polypeptides (mammals). In Leigh's disease, there may be an abnormality or deficiency of cytochrome oxidase.
Test method:Colorimetric
Precautions:
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Alternative:
PTGS2
COX-2
COX2
GRIPGHS
PGG/HS
PGHS-2
PHS-2
hCox-2
cyclooxygenase 2b
prostaglandin G/H synthase and cyclooxygenase
prostaglandin-endoperoxide synthase 2
Storage conditions:The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Delivery conditions:Gel pack with blue ice.
Usage:
• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Sensitivity:0.114 ng/mL
Sample type:
Cell culture supernatants
Serum
Plasma
Other biological fluids
Assay length:Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components:
• Rat Cyclooxygenase-2 microplate
• Rat Cyclooxygenase-2 standard
• Rat Cyclooxygenase-2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Calibration range:0.312 - 20ng/mL